Digoxin-like immunoreactivity, displacement of ouabain and inhibition of Na+/K+ ATPase by four steroids known to be increased in essential hypertension
| Autor: | Morris R. Pudek, Karin H. Humphries, David W. Seccombe, Wojciech Nowaczynski |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Digoxin
medicine.medical_specialty ATPase Clinical Biochemistry Endogeny In Vitro Techniques Kidney Essential hypertension Ouabain Natriuresis Dogs Internal medicine medicine Animals Na+/K+-ATPase chemistry.chemical_classification biology Immune Sera Blood Proteins General Medicine Saponins medicine.disease Cardenolides Enzyme Endocrinology chemistry Hypertension biology.protein Steroids Sodium-Potassium-Exchanging ATPase medicine.drug |
| Zdroj: | Clinical Biochemistry. 22:17-21 |
| ISSN: | 0009-9120 |
| DOI: | 10.1016/s0009-9120(89)80064-5 |
| Popis: | An endogenous digoxin-like immunoreactive substance(s) (DLIS, “endoxin”) may be of significance in the etiology of essential hypertension (EH). Progesterone, dehydroepiandrosterone sulphate (DHEA-S), 11-deoxycortisol and 18-hydroxy-11-deoxycorticosterone (18-OH-DOC), four steroids known to be increased in essential hypertension, were found to have digoxin-like immunoreactivity at levels 1,000 times higher than physiological concentrations. Of these steroids, progesterone and 18-OH-DOC were the most efficient in displacing 3 H-ouabain from canine kidney Na + /K + ATPase whereas progesterone and 11-deoxycortisol were the most potent inhibitors of this enzyme's activity. Although 18-OH-DOC and DHEA-S cross-reacted with digoxin-specific antibodies, their ability to inhibit Na + /K + ATPase activity was minimal. Although it is concluded that these steroids may contribute to DLIS as isolated from hypertensive patients, it is unlikely that they would be of physiological significance in the etiology of EH unless they were to accumulate and act synergistically within vascular wall smooth muscle tissues. |
| Databáze: | OpenAIRE |
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