Calcium is sufficient but not necessary for activation of sheep platelet cytosolic phospholipase A2
| Autor: | Kelly K. Kruszka, Lori A. Zupan, Richard W. Gross |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Blood Platelets
Biophysics chemistry.chemical_element Calcium Phospholipase Sodium Chloride Biochemistry Phospholipases A Plasmalogen chemistry.chemical_compound Phospholipase A2 Structural Biology Genetics Extracellular Animals Molecular Biology Phospholipids Phospholipase A Sheep biology Platelet Cell Biology Enzyme Activation Cytosol Phospholipases A2 chemistry Arachidonic acid biology.protein Chromatography Gel Intracellular |
| Zdroj: | FEBS Letters. (1):27-30 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(91)80753-P |
| Popis: | In this study we demonstrate that: (1) although the major phospholipase A2 present in sheep platelets is activated by calcium ions, it can effectively catalyze hydrolysis of the sn-2 ester linkage in phospholipids in the absence of calcium: (2) expression of calcium-independent phospholipase A2 activity can be induced by NACl utilizing purified (but not crude) cytosolic enzyme; and (3) calcium-independent phospholipase A2 activity is regulated by a reconstitutable cytosolic protein. Collectively, these results underscore the fundamental catalytic differences between extracellular and intracellular calcium-dependent phospholipases A2 and demonstrate that calcium is sufficient, but not necessary, for the activation of this class of intracellular phospholipases A2 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|