A Unique Mutation in a MYB Gene Cosegregates with the Nectarine Phenotype in Peach
| Autor: | Laura Gazza, Ignazio Verde, Stefano Tartarini, Daniele Bassi, Laura Rossini, Francesco Strozzi, Igor Pacheco, G. Pea, Maria Teresa Dettori, Elisa Vendramin, Luca Dondini, Simone Scalabrin |
|---|---|
| Přispěvatelé: | Elisa Vendramin, Giorgio Pea, Luca Dondini, Igor Pacheco, Maria Teresa Dettori, Laura Gazza, Simone Scalabrin, Francesco Strozzi, Stefano Tartarini, Daniele Bassi, Ignazio Verde, Laura Rossini |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Fine mapping
Agricultural Biotechnology Plant Science Breeding Plant Genetics Polymerase Chain Reaction Trees Exon Genotype Plant Genomics MYB Genetics Plant Growth and Development Multidisciplinary Chromosome Mapping Agriculture Cell Differentiation Trichomes Genomics Plants G Locu Phenotype Medicine Prunus Research Article Genetic Markers medicine.medical_specialty Marker-Assisted Selection Retroelements Science SNP Locus (genetics) Crops Biology Genes Plant Chromosomes Plant Fruits Genetic linkage Genome Analysis Tools Molecular genetics medicine Trait Locus Analysis Gene Crosses Genetic DNA Primers Crop Genetics Linkage Maps Chromosome Genetic Maps Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 3, p e90574 (2014) |
| Popis: | Nectarines play a key role in peach industry; the fuzzless skin has implications for consumer acceptance. The peach/nectarine (G/g) trait was described as monogenic and previously mapped on chromosome 5. Here, the position of the G locus was delimited within a 1.1 cM interval (635 kb) based on linkage analysis of an F2 progeny from the cross 'Contender' (C, peach) x 'Ambra' (A, nectarine). Careful inspection of the genes annotated in the corresponding genomic sequence (Peach v1.0), coupled with variant discovery, led to the identification of MYB gene PpeMYB25 as a candidate for trichome formation on fruit skin. Analysis of genomic re-sequencing data from five peach/nectarine accessions pointed to the insertion of a LTR retroelement in exon 3 of the PpeMYB25 gene as the cause of the recessive glabrous phenotype. A functional marker (indelG) developed on the LTR insertion cosegregated with the trait in the CxA F2 progeny and was validated on a broad panel of genotypes, including all known putative donors of the nectarine trait. This marker was shown to efficiently discriminate between peach and nectarine plants, indicating that a unique mutational event gave rise to the nectarine trait and providing a useful diagnostic tool for early seedling selection in peach breeding programs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|