Engineering a High-Affinity Anti-Methoxy Poly(ethylene glycol) (mPEG) Antibody for Sensitive Immunosensing of mPEGylated Therapeutics and mPEG Molecules

Autor:	Chiao-Yu Hsiao, Jun-Lun Meng, Jung-Zhe Hong, Xuan-Huong Ly, Meng-Hsuan Lin, Chin-Yuan Chang, Minh-Tram T. Nguyen, Tian-Lu Cheng, Wen-Wei Lin, Pierre-Alain Burnouf, Talal Salem Al-Qaisi, En-Shuo Liu, Yu-Cheng Su
Rok vydání:	2022
Předmět:	Molecular Weight Pharmacology Organic Chemistry Biomedical Engineering Pharmaceutical Science Bioengineering Antibodies Polyethylene Glycols Biotechnology
Zdroj:	Bioconjugate Chemistry. 33:2180-2188
ISSN:	1520-4812 1043-1802
DOI:	10.1021/acs.bioconjchem.2c00416
Popis:	Sensitive quantification of methoxy poly(ethylene glycol) (mPEG)-conjugated therapeutics for pharmacokinetic determination is critical for mPEGylated drug development. However, sensitive measurement of low-molecular-weight (lmw) mPEG compounds remains challenging due to epitope competition between backbone-specific anti-PEG antibodies. Here, we engineered a high-affinity methoxy-specific anti-mPEG antibody for sensitive quantification of free mPEG molecules and mPEGylated therapeutics. The affinity-enhanced h15-2Y antibody variant shows a 10.3-fold increase in mPEG-binding activity compared to parental h15-2b. h15-2Y-based sandwich ELISA can effectively quantify lmw mPEG
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d144a696e7eefb84565ac899eb24ce2 https://doi.org/10.1021/acs.bioconjchem.2c00416 Zobrazit plný text záznamu