Engineering a High-Affinity Anti-Methoxy Poly(ethylene glycol) (mPEG) Antibody for Sensitive Immunosensing of mPEGylated Therapeutics and mPEG Molecules
Autor: | Chiao-Yu Hsiao, Jun-Lun Meng, Jung-Zhe Hong, Xuan-Huong Ly, Meng-Hsuan Lin, Chin-Yuan Chang, Minh-Tram T. Nguyen, Tian-Lu Cheng, Wen-Wei Lin, Pierre-Alain Burnouf, Talal Salem Al-Qaisi, En-Shuo Liu, Yu-Cheng Su |
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Rok vydání: | 2022 |
Předmět: | |
Zdroj: | Bioconjugate Chemistry. 33:2180-2188 |
ISSN: | 1520-4812 1043-1802 |
Popis: | Sensitive quantification of methoxy poly(ethylene glycol) (mPEG)-conjugated therapeutics for pharmacokinetic determination is critical for mPEGylated drug development. However, sensitive measurement of low-molecular-weight (lmw) mPEG compounds remains challenging due to epitope competition between backbone-specific anti-PEG antibodies. Here, we engineered a high-affinity methoxy-specific anti-mPEG antibody for sensitive quantification of free mPEG molecules and mPEGylated therapeutics. The affinity-enhanced h15-2Y antibody variant shows a 10.3-fold increase in mPEG-binding activity compared to parental h15-2b. h15-2Y-based sandwich ELISA can effectively quantify lmw mPEG |
Databáze: | OpenAIRE |
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