The role of pruriceptors in enhancing sensitivity to pruritogens in a murine chronic compression model of dorsal root ganglion
| Autor: | Chao Ma, Tao Wang, Jin Tao, Yehong Fang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
TRPV1 Pain TRPV Cation Channels Mice Transgenic Pharmacology Hyperkinesis Itch lcsh:RC346-429 Receptors G-Protein-Coupled Cellular and Molecular Neuroscience chemistry.chemical_compound Histamine receptor Calcium imaging Dorsal root ganglion Ganglia Spinal medicine Animals RNA Messenger Receptors Histamine H1 Receptor Molecular Biology Sensitization lcsh:Neurology. Diseases of the nervous system Receptors Histamine H4 Neurons Behavior business.industry Nerve Compression Syndromes Pruritus Research Peptide Fragments Up-Regulation Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure chemistry nervous system Capsaicin Chronic Disease Calcium Cattle Pruriceptor business Histamine DRG compression |
| Zdroj: | Molecular Brain, Vol 14, Iss 1, Pp 1-9 (2021) Molecular Brain |
| ISSN: | 1756-6606 |
| Popis: | Chronic pruritus is a symptom that commonly observed in neurological diseases. It has been hypothesized that the chronic pruritus may result from sensitization of itch-signaling pathways but the mechanisms remain obscure. In this study, we established a mouse model of chronic compression of dorsal root ganglion (CCD) and injected various pruritogenic and algogenic agents intradermally to the calf skin ipsilateral to the compressed dorsal root ganglion (DRG). Compared to the naïve mice, a significant increase in itch-related behaviors was observed in the CCD mice after the injection of pruritogens including histamine and BAM8-22, but not after the injection of capsaicin, although all the above agents evoked enhanced pain-related behaviors toward the injected site. In addition, we investigated if pruritogen-evoked activities of DRG neurons are enhanced in this model. In vivo calcium imaging revealed that compressed DRG neurons exhibited significant enhanced responses to histamine and BAM8-22. Immunoflorescent staining also showed that the histamine receptor H1 and the capsaicin receptor TRPV1 were significantly upregulated in DRG neurons. Our findings indicated that in mice modeling the sensitization of primary pruriceptive neurons may underlie the enhanced itch sensation after chronic compression of DRG neurons, and may play a role in chronic pruritus in neurological diseases. |
| Databáze: | OpenAIRE |
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