Immunoperoxidase localization of human papillomavirus in laryngeal papillomas
| Autor: | Howard G. Smith, Gerald B. Healy, Bennett Jenson, Franklin Pass, Ernest E. Lack, Gordon F. Vawter |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Larynx
Serotype Pathology medicine.medical_specialty Biology Inclusion Bodies Viral Immunoenzyme Techniques Antigen Virology medicine Humans Human papillomavirus Antigens Viral Laryngeal Neoplasms Papillomaviridae Immunoperoxidase Papilloma virus diseases medicine.disease Laryngeal papillomas Microscopy Electron Infectious Diseases medicine.anatomical_structure Immunohistochemistry |
| Zdroj: | Intervirology. 14(3-4) |
| ISSN: | 0300-5526 |
| Popis: | Five antigenically distinct serotypes of human papillomavirus (HPV) cause various cutaneous verrucae with distinct clinical features. Laryngeal and other mucosal papillomas, also suspected to have a viral etiology, were examined for the presence of a genus-specific (common) antigen of HPV using a peroxidase-antiperoxidase (PAP) technique. The primary antiserum used was reactive against laryngeal papillomas from 26 of 35 randomly selected patients (74%); overall, 49 of the 102 squamous papillomas stained were positive for HPV (48%). HPV antigen was not demonstrated within frankly dysplastic squamous cells. Nuclear localization of virus was confirmed by electron microscopy in 3 cases positive for HPV. PAP localization of HPV is rapid and more efficient than routine electron microscopy. Our data indicate a common pathogenesis for some papillomas and cutaneous verrucae and suggest a future role for immunohistochemistry in evaluating the epidemiology and pathobiology of laryngeal papillomatosis. |
| Databáze: | OpenAIRE |
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