Effects of Dimethyl Sulfoxide on the Pluripotency and Differentiation Capacity of Mouse Embryonic Stem Cells
| Autor: | Sung Hyun Kim, Zae-Young Ryoo, Song Park, Mee-Hyun Lee, Hai Huang, Jae Jung Ha, Junkoo Yi, Myoungok Kim, Si-Jun Park, Dae-Hyun Kim |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pluripotent Stem Cells
0301 basic medicine Cell Survival medicine.medical_treatment Biology Leukemia Inhibitory Factor Mice 03 medical and health sciences chemistry.chemical_compound medicine Animals Dimethyl Sulfoxide Cells Cultured 030102 biochemistry & molecular biology Oncogene Dimethyl sulfoxide Cell Cycle Gene Expression Regulation Developmental Cell Differentiation Mouse Embryonic Stem Cells Cell Biology Methylation DNA Methylation Embryonic stem cell Cell biology 030104 developmental biology Cytokine chemistry embryonic structures FOXA2 Stem cell Leukemia inhibitory factor Biomarkers Developmental Biology Biotechnology |
| Zdroj: | Cellular Reprogramming. 22:244-253 |
| ISSN: | 2152-4998 2152-4971 |
| DOI: | 10.1089/cell.2020.0006 |
| Popis: | Mouse embryonic stem cells (mESCs) go through self-renewal in the existence of the cytokine leukemia inhibitory factor (LIF). LIF is added to the mouse stem cells culture medium, and its removal results in fast differentiation. Dimethyl sulfoxide (DMSO) is one of the most used solvents in drug test. We exposed 4-day mESC cultures to different concentrations of DMSO (0.1%, 0.5%, 1.0%, and 2.0%) to identify the safest dose exhibiting efficacy as a solvent. mESCs grown under general pluripotency conditions in the absence of LIF were treated with DMSO. In addition, as a control for differentiation, mESCs were grown in the absence of LIF. DMSO upregulated the mRNA expression level of pluripotency markers. Moreover, DMSO reduced the mRNA expression levels of ectodermal marker (β-tubulin3), mesodermal marker (Hand1), and endodermal markers (Foxa2 and Sox17) in mESCs. These results indicate that DMSO treatment enhances the pluripotency and disrupts the differentiation of mESCs. We also show that members of the Tet oncogene family are critical to inhibiting the differentiation and methylation of mESCs. DMSO is appropriate to sustain the pluripotency of mESCs in the absence of LIF, and that mESCs can be sustained in an undifferentiated state using DMSO. Therefore, DMSO may, in part, function as a substitute for LIF. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|