In vivo effects of 17α-ethinylestradiol, 17β-estradiol and 4-nonylphenol on insulin-like growth-factor binding proteins (igfbps) in Atlantic salmon

Autor: Tara A. Duffy, Stephen D. McCormick, Jason P. Breves, Ingibjörg Eir Einarsdottir, Björn Thrandur Björnsson
Rok vydání: 2018
Předmět:
0301 basic medicine
medicine.medical_specialty
Health
Toxicology and Mutagenesis
medicine.medical_treatment
Salmo salar
Estrogen receptor
Growth hormone receptor
010501 environmental sciences
Aquatic Science
Endocrine Disruptors
Ethinyl Estradiol
01 natural sciences
Insulin-like growth factor-binding protein
03 medical and health sciences
chemistry.chemical_compound
Insulin-like growth factor
Vitellogenin
Vitellogenins
Phenols
Internal medicine
medicine
Endocrine system
Animals
RNA
Messenger
Salmo
Insulin-Like Growth Factor I
0105 earth and related environmental sciences
biology
Estradiol
Chemistry
Body Weight
Gene Expression Regulation
Developmental
biology.organism_classification
Nonylphenol
Insulin-Like Growth Factor Binding Proteins
030104 developmental biology
Endocrinology
Branchial Region
Liver
Growth Hormone
biology.protein
hormones
hormone substitutes
and hormone antagonists
Water Pollutants
Chemical
Zdroj: Aquatic toxicology (Amsterdam, Netherlands). 203
ISSN: 1879-1514
Popis: Feminizing endocrine disrupting compounds (EDCs) affect the growth and development of teleost fishes. The major regulator of growth performance, the growth hormone (Gh)/insulin-like growth-factor (Igf) system, is sensitive to estrogenic compounds and mediates certain physiological and potentially behavioral consequences of EDC exposure. Igf binding proteins (Igfbps) are key modulators of Igf activity, but their alteration by EDCs has not been examined. We investigated two life-stages (fry and smolts) of Atlantic salmon (Salmo salar), and characterized how the Gh/Igf/Igfbp system responded to waterborne 17α-ethinylestradiol (EE2), 17β-estradiol (E2) and 4-nonylphenol (NP). Fry exposed to EE2 and NP for 21 days had increased hepatic vitellogenin (vtg) mRNA levels while hepatic estrogen receptor α (erα), gh receptor (ghr), igf1 and igf2 mRNA levels were decreased. NP-exposed fry had reduced body mass and total length compared to controls. EE2 and NP reduced hepatic igfbp1b1, -2a, -2b1, -4, -5b2 and -6b1, and stimulated igfbp5a. In smolts, hepatic vtg mRNA levels were induced following 4-day exposures to all three EDCs, while erα only responded to EE2 and E2. EDC exposures did not affect body mass or fork length; however, EE2 diminished plasma Gh and Igf1 levels in parallel with reductions in hepatic ghr and igf1. In smolts, EE2 and E2 diminished hepatic igfbp1b1, -4 and -6b1, and stimulated igfbp5a. There were no signs of compromised ionoregulation in smolts, as indicated by unchanged branchial ion pump/transporter mRNA levels. We conclude that hepatic igfbps respond (directly and/or indirectly) to environmental estrogens during two key life-stages of Atlantic salmon, and thus may modulate the growth and development of exposed individuals.
Databáze: OpenAIRE
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