Red fruit (Pandanus conoideus Lam) oil stimulates nitric oxide production and reduces oxidative stress in endothelial cells
| Autor: | Huige Li, Solveig Hasselwander, Christian Schirra, Ning Xia, Ulrich Förstermann |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Endothelial cells Medicine (miscellaneous) Vasodilation Mouse aorta 030204 cardiovascular system & hematology Pharmacology medicine.disease_cause Nitric oxide 03 medical and health sciences chemistry.chemical_compound Pandanus conoideus Lam 0302 clinical medicine Enos medicine TX341-641 Nutrition and Dietetics biology Nutrition. Foods and food supply Pandanus conoideus biology.organism_classification Comet assay 030104 developmental biology chemistry Oxidative stress Phosphorylation Endothelial nitric oxide synthase Reactive oxygen species Food Science |
| Zdroj: | Journal of Functional Foods, Vol 51, Iss, Pp 65-74 (2018) |
| ISSN: | 1756-4646 |
| DOI: | 10.1016/j.jff.2018.10.014 |
| Popis: | Red fruit oil (RFO) is used in traditional medicine for the treatment of a number of diseases. However, evidence for the biological effects and action mechanisms is still lacking. In the present study, we show for the first time that RFO stimulated the phosphorylation of the endothelial nitric oxide synthase (eNOS) and enhanced the NO production in human endothelial cells. In isolated mouse aorta, RFO induced a vasodilation, with a significant effect evident at a concentration as low as 1:100,000 dilution. The RFO-induced vasodilation could be completely prevented by eNOS inhibition, indicating that RFO contains highly potent substances stimulating eNOS activity. In addition, RFO reduced oxidative stress in endothelial cells, as demonstrated with three different methods. In the comet assay, RFO significantly decreased oxidative DNA damage induced by genotoxicants. These results suggest that enhanced endothelial NO production and reduced oxidative stress may represent part of the molecular mechanisms responsible for the therapeutic effects of RFO. |
| Databáze: | OpenAIRE |
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