Kinetic Microplate-Based Assays for Inhibitors of Mitochondrial NADH:Ubiquinone Oxidoreductase (Complex I) and Succinate:Cytochrome c Oxidoreductase
| Autor: | A.L. Devonshire, P.J. Jewess |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Submitochondrial Particles
Drug Evaluation Preclinical Biophysics In Vitro Techniques Mitochondrion Biochemistry Electron transfer Oxidoreductase NAD(P)H Dehydrogenase (Quinone) Animals Submitochondrial particle Enzyme Inhibitors Molecular Biology chemistry.chemical_classification biology Diptera Cytochrome c Reproducibility of Results Cell Biology In vitro Mitochondria Muscle Kinetics Enzyme chemistry Evaluation Studies as Topic Spectrophotometry Coenzyme Q – cytochrome c reductase biology.protein Succinate Cytochrome c Oxidoreductase |
| Zdroj: | Analytical Biochemistry. 272:56-63 |
| ISSN: | 0003-2697 |
| Popis: | Kinetic microplate-based assays for both mitochondrial NADH:ubiquinone oxidoreductase (complex I) and succinate:cytochrome c oxidoreductase using insect submitochondrial particles as the source of the enzyme activities have been developed. These assays have been used to design high-throughput screens for inhibitors of these mitochondrial electron transfer activities to assess their intrinsic in vitro efficacies as potential pesticides. These methods can be used to test up to 60 compounds per day without the use of automated sample handling and diluting technology. The accuracy, specificity, and reproducibility of the microplate methods compared well with conventional spectrophotometer-based assays. |
| Databáze: | OpenAIRE |
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