Inhibition of microRNA-183 expression resists human umbilical vascular endothelial cells injury by upregulating expression of IRS1
| Autor: | Bo Yu, Dandan Liu, Yefei Zhan, Yingying Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
endocrine system
Angiogenesis IRS1 Cell Survival Pharmaceutical Science Inflammation Apoptosis 02 engineering and technology medicine.disease_cause 030226 pharmacology & pharmacy 03 medical and health sciences 0302 clinical medicine microRNA medicine Human Umbilical Vein Endothelial Cells Humans MicroRNA-183 Cells Cultured oxidized low-density lipoprotein human umbilical vascular endothelial cells Chemistry lcsh:RM1-950 General Medicine Cell cycle 021001 nanoscience & nanotechnology Up-Regulation Lipoproteins LDL MicroRNAs Oxidative Stress lcsh:Therapeutics. Pharmacology embryonic structures Cancer research cardiovascular system Insulin Receptor Substrate Proteins medicine.symptom 0210 nano-technology Oxidative stress Lipoprotein Research Article cell injury |
| Zdroj: | Drug Delivery Drug Delivery, Vol 26, Iss 1, Pp 612-621 (2019) |
| ISSN: | 1521-0464 1071-7544 |
| Popis: | Our study aims to investigate the effect of microRNA-183 (miR-183) on human umbilical vascular endothelial cells (HUVECs) injury by targeting IRS1. HUVECs injury was induced by oxidized low-density lipoprotein (ox-LDL). HUVECs were grouped so as to explore the role of ox-LDL and miR-183 in HUVECs injury, with the expression of miR-183 and IRS1 detected. Additionally, the related factors of oxidative stress and inflammation, as well as angiogenesis ability, proliferation, cell cycle, apoptosis, invasion, and migration abilities were also measured. Ox-LDL treatment could decrease the activity of HUVECs, increase the level of oxidative stress and inflammation, and induce the HUVECs injury. miR-183 could inhibit the expression of IRS1. The inhibition of miR-183 expression in ox-LDL-induced HUVECs injury could enhance cell activity, inhibit inflammatory level, and thus resist cell injury. Low expression of IRS1 could reverse the inhibition of miR-183 on HUVECs injury. This study highlights that inhibition of miR-183 expression may resist HUVECs injury by upregulating expression of IRS1. |
| Databáze: | OpenAIRE |
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