The small regulatory RNA molecule MicA is involved in Salmonella enterica serovar Typhimurium biofilm formation
| Autor: | Sigrid C. J. De Keersmaecker, Jos Vanderleyden, Kathleen Marchal, Gwendoline Kint, David De Coster |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Salmonella typhimurium
Microbiology (medical) DOWN-REGULATION ENVELOPE STRESS Mutant lcsh:QR1-502 Biology medicine.disease_cause ANTISENSE RNA Microbiology lcsh:Microbiology HFQ chemistry.chemical_compound Bacterial Proteins medicine OUTER-MEMBRANE PROTEINS Escherichia coli Science & Technology Biofilm Wild type Biology and Life Sciences RNA food and beverages Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition biology.organism_classification Autoinducer-2 Cell biology AUTOINDUCER-2 Carbon-Sulfur Lyases RNA Bacterial Quorum sensing stomatognathic diseases chemistry Salmonella enterica ESCHERICHIA-COLI NONCODING RNAS Biofilms BACTERIA RNA Small Untranslated bacteria LUXS sense organs Life Sciences & Biomedicine Research Article |
| Zdroj: | BMC MICROBIOLOGY BMC Microbiology BMC Microbiology, Vol 10, Iss 1, p 276 (2010) |
| ISSN: | 1471-2180 |
| Popis: | Background LuxS is the synthase enzyme of the quorum sensing signal AI-2. In Salmonella Typhimurium, it was previously shown that a luxS deletion mutant is impaired in biofilm formation. However, this phenotype could not be complemented by extracellular addition of quorum sensing signal molecules. Results Analysis of additional S. Typhimurium luxS mutants indicated that the LuxS enzyme itself is not a prerequisite for a wild type mature biofilm. However, in close proximity of the luxS coding sequence, a small RNA molecule, MicA, is encoded on the opposite DNA strand. Interference with the MicA expression level showed that a balanced MicA level is essential for mature Salmonella biofilm formation. Several MicA targets known to date have previously been reported to be implicated in biofilm formation in Salmonella or in other bacterial species. Additionally, we showed by RT-qPCR analysis that MicA levels are indeed altered in some luxS mutants, corresponding to their biofilm formation phenotype. Conclusions We show that the S. Typhimurium biofilm formation phenotype of a luxS mutant in which the complete coding region is deleted, is dependent on the sRNA molecule MicA, encoded in the luxS adjacent genomic region, rather than on LuxS itself. Future studies are required to fully elucidate the role of MicA in Salmonella biofilm formation. |
| Databáze: | OpenAIRE |
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