Leishmania spp. Epidemiology of Canine Leishmaniasis in the Yucatan Peninsula
Autor: | Manuel Emilio Bolio-González, Manuel Sánchez-Moreno, Mario Barrera-Pérez, Clotilde Marín, Carlos H. Sauri-Arceo, Roger Iván Rodríguez-Vivas, Silvia S. Longoni, Angeles López-Cespedes, Francisco Javier Escobedo-Ortegón |
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Jazyk: | angličtina |
Rok vydání: | 2012 |
Předmět: |
Antibodies
Protozoan lcsh:Medicine Infantum lcsh:Technology Diagnosis Prevalence Canine leishmaniasis Parasite hosting Dog Diseases lcsh:Science Leishmaniasis General Environmental Science Leishmania Visceral Leishmaniasis education.field_of_study Coinfection Host General Medicine Research Article Article Subject Blotting Western Population Antigens Protozoan Enzyme-Linked Immunosorbent Assay Superoxide dismutase Biology Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology Microbiology Dogs Cutaneous leishmaniasis parasitic diseases medicine Animals education Mexico Superoxide Dismutase lcsh:T lcsh:R medicine.disease biology.organism_classification Virology Visceral leishmaniasis Viannia braziliensis biology.protein lcsh:Q |
Zdroj: | The Scientific World Journal, Vol 2012 (2012) Digibug. Repositorio Institucional de la Universidad de Granada instname The Scientific World Journal |
Popis: | Canine Leishmaniasis is widespread in various Mexican states, where different species ofLeishmaniahave been isolated from dogs. In the present study, we describe the detection ofL. braziliensis, L. infantum,andL. mexicanain serum of dogs from the states of Yucatan and Quintana Roo in the Yucatan Peninsula (Mexico). A total of 412 sera were analyzed by ELISA using the total extract of the parasite and the iron superoxide dismutase excreted by different trypanosomatids as antigens. We found the prevalence ofL. braziliensisto be 7.52%,L. infantumto be 6.07%, andL. mexicanato be 20.63%, in the dog population studied. The results obtained with ELISA using iron superoxide dismutase as the antigen were confirmed by western blot analysis with its greater sensitivity, and the agreement between the two techniques was very high. |
Databáze: | OpenAIRE |
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