Comparison of nasopharyngeal flocked swabs and nasopharyngeal wash collection methods for respiratory virus detection in hospitalized children using real-time polymerase chain reaction
| Autor: | Thomas W. Hennessy, Karen Miernyk, Lori Chikoyak, Rosalyn J. Singleton, Kimberlee Boyd Hummel, Lisa R. Bulkow, Carolynn DeByle |
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| Rok vydání: | 2012 |
| Předmět: |
Male
viruses animal diseases Real-Time Polymerase Chain Reaction Sensitivity and Specificity Article Specimen Handling Nasopharyngeal Wash stomatognathic system Nasopharynx Virology Humans Medicine Molecular diagnostic techniques Respiratory Tract Infections Collection methods Respiratory tract infections business.industry Infant Newborn Infant Infant newborn Real-time polymerase chain reaction Molecular Diagnostic Techniques Child Preschool Respiratory virus Female business Child Hospitalized |
| Zdroj: | Journal of Virological Methods |
| ISSN: | 0166-0934 |
| Popis: | This paper describes the molecular detection of respiratory viruses from nasopharyngeal flocked swabs (flocked swabs) and nasopharyngeal washes (washes) in a clinical setting. Washes and flocked swabs collected from children3 years old hospitalized with a lower respiratory tract infection were tested for parainfluenza virus 1-3, respiratory syncytial virus, influenza A and B and metapneumovirus (Group 1) and adenovirus, rhinovirus and coronavirus (Group 2) using real-time reverse transcriptase PCR (rRT-PCR). A consensuses standard was used to determine sensitivity and compare cycle thresholds (C(T)) of washes and flocked swabs for each virus and group of viruses. Sensitivities ranged from 79 to 89% and 69 to 94% for flocked swabs and washes, respectively, excluding AdV which had a sensitivity of 35% for flocked swabs. When the flocked swabs and washes of Group 1 viruses were collected on the day of admission, the sensitivity of both sample types was 100%. Wash specimens had a lower C(T) value and higher sensitivity than flocked swabs; however there was no statistical difference in the sensitivity of a flocked swab (89%) versus wash (93%) for the detection of Group 1 viruses, particularly when samples were collected on the same day. Flocked swabs may be a useful alternative to washes for detection of respiratory viruses in clinical settings. |
| Databáze: | OpenAIRE |
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