Systems analysis identifies an essential role for SHANK-associated RH domain-interacting protein (SHARPIN) in macrophage Toll-like receptor 2 (TLR2) responses
| Autor: | Antti Niemistö, Alan Aderem, M. Kristina Hamilton, Jacques J. Peschon, Owen M. Siggs, Daniel E. Zak, Kathleen A. Kennedy, Shannon G. Fallen, Bruce Beutler, Rosa Suen, Elizabeth S. Gold, Joe S. Valvo, Frank Schmitz, Carrie D. Johnson, Tetyana Stolyar, Alan H. Diercks, Irina Podolsky |
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| Rok vydání: | 2011 |
| Předmět: |
Systems Analysis
Biology medicine.disease_cause Mice Protein Interaction Mapping IKBKG medicine Animals SHARPIN Gene Transcription factor DNA Primers Mice Knockout Toll-like receptor Mutation Multidisciplinary Base Sequence Macrophages Systems Biology Intracellular Signaling Peptides and Proteins NF-kappa B Biological Sciences Molecular biology Immunity Innate Toll-Like Receptor 2 Cell biology Mice Inbred C57BL Transcription Factor AP-1 TLR2 IκBα Signal transduction Carrier Proteins Signal Transduction |
| Zdroj: | Proceedings of the National Academy of Sciences. 108:11536-11541 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1107577108 |
| Popis: | Precise control of the innate immune response is essential to ensure host defense against infection while avoiding inflammatory disease. Systems-level analyses of Toll-like receptor (TLR)-stimulated macrophages suggested that SHANK-associated RH domain-interacting protein (SHARPIN) might play a role in the TLR pathway. This hypothesis was supported by the observation that macrophages derived from chronic proliferative dermatitis mutation ( cpdm ) mice, which harbor a spontaneous null mutation in the Sharpin gene, exhibited impaired IL-12 production in response to TLR activation. Systems biology approaches were used to define the SHARPIN-regulated networks. Promoter analysis identified NF-κB and AP-1 as candidate transcription factors downstream of SHARPIN, and network analysis suggested selective attenuation of these pathways. We found that the effects of SHARPIN deficiency on the TLR2-induced transcriptome were strikingly correlated with the effects of the recently described hypomorphic L153P/ panr2 point mutation in Ikbkg [ N F-κB E ssential Mo dulator (NEMO)], suggesting that SHARPIN and NEMO interact. We confirmed this interaction by co-immunoprecipitation analysis and furthermore found it to be abrogated by panr2. NEMO-dependent signaling was affected by SHARPIN deficiency in a manner similar to the panr2 mutation, including impaired p105 and ERK phosphorylation and p65 nuclear localization. Interestingly, SHARPIN deficiency had no effect on IκBα degradation and on p38 and JNK phosphorylation. Taken together, these results demonstrate that SHARPIN is an essential adaptor downstream of the branch point defined by the panr2 mutation in NEMO. |
| Databáze: | OpenAIRE |
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