Simple method for the routine determination of betaine and N,N-dimethylglycine in blood and urine
| Autor: | Sandra Pawliczek, Maurice D. Laryea, Udo Wendel, Folkert Steinhagen |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
Detection limit Chromatography Biochemistry (medical) Clinical Biochemistry Infant Sarcosine Urine Middle Aged Sensitivity and Specificity High-performance liquid chromatography Betaine Dimethylglycine chemistry.chemical_compound chemistry Reference Values Bromide Child Preschool Humans Spectrophotometry Ultraviolet Child Derivatization Quantitative analysis (chemistry) Chromatography High Pressure Liquid |
| Zdroj: | Clinical Chemistry. 44:1937-1941 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/44.9.1937 |
| Popis: | A simple and convenient method using commercially available derivatization reagents is described for the measurement of betaine and N,N-dimethylglycine (DMG) in blood and urine. Precolumn derivatization of plasma or urine is performed directly in acetonitrile without extraction with p-bromophenacyl bromide and crown ether as catalyst. The p-bromophenacyl ester derivatives are then separated by high-performance liquid chromatography, using an isocratic system of acetonitrile and water containing choline. Effluent was monitored at 254 nm. The limit of detection was 5 μmol/L for betaine and 2 μmol/L for DMG. Analytical recovery was >97% for both analytes. Total and within-day CVs were 2.0–4.4% and 0.9–2.2% for DMG. For betaine, the total and within-day CVs were 1.3–5.3% and 0.4–3.8%, respectively. The method is precise and cost-effective and has been used successfully to determine the concentrations of DMG and betaine in human plasma and urine. |
| Databáze: | OpenAIRE |
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