Alga-Made Anti-Hepatitis B Antibody Binds to Human Fcγ Receptors

Autor: Muriel Bardor, Franziska Hempel, Szymon Stelter, Uwe G. Maier, Patrice Lerouge, Julian K.-C. Ma, Gaëtan Vanier, Jessica Vanier
Přispěvatelé: Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), St George's, University of London, LOEWE Center for Synthetic Microbiology (SYNMIKRO), Philipps Universität Marburg, Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.)
Rok vydání: 2017
Předmět:
0301 basic medicine
Glycosylation
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
THP-1 Cells
medicine.drug_class
Oligosaccharides
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Monoclonal antibody
Applied Microbiology and Biotechnology
law.invention
03 medical and health sciences
law
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
medicine
Humans
Phaeodactylum tricornutum
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Receptor
Opsonin
ComputingMilieux_MISCELLANEOUS
[SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis
Diatoms
Antibody-dependent cell-mediated cytotoxicity
Hepatitis B Surface Antigens
biology
Chemistry
Ligand binding assay
Receptors
IgG
Antibodies
Monoclonal
[SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular Networks [q-bio.MN]
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
General Medicine
biology.organism_classification
Recombinant Proteins
Culture Media
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
3. Good health
[SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding
HEK293 Cells
[CHIM.POLY]Chemical Sciences/Polymers
030104 developmental biology
Biochemistry
Recombinant DNA
biology.protein
Molecular Medicine
Asparagine
Antibody
Zdroj: Biotechnology Journal
Biotechnology Journal, Wiley-VCH Verlag, 2017, 13, pp.1700496. ⟨10.1002/biot.201700496]⟩
ISSN: 1860-6768
1860-7314
Popis: Microalgae are unicellular eukaryotic organisms which represent an emerging alternative to other cell biofactories commonly used to produce monoclonal antibodies. Microalgae display several biotechnological advantages such as their rapid growth rate and their phototrophic lifestyle allowing low production costs as protein expression is solar-fueled. Recently, a fully assembled recombinant IgG antibody directed against Hepatitis B surface antigen is produced and secreted in the culture medium of the diatom Phaeodactylum tricornutum. A biochemical characterization of this recombinant antibody demonstrated that the Asn-297 is N-glycosylated by oligomannosides. In the immune system, antibodies interact with effector molecules and cells through their Fc part and the recognition of Fcγ receptors (FcγR) which are important for inducing phagocytosis of opsonized microbes. Interactions between IgG and FcγR are influenced by the N-glycan structures present on the Asn-297. In this study, the authors characterized the binding capacity of the anti-hepatitis B recombinant IgG produced in P. tricornutum to two human Fcγ receptors (FcγRI and IIIa) using a cellular binding assay and surface plasmon resonance (SPR). This allowed us to demonstrate that the alga-made antibody is able to bind FcγRI with a reduced affinity and engages FcyRIIIa with 3-times higher affinity compared to a control human IgG1.
Databáze: OpenAIRE
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