Confirmation of a Campylobacteriosis Outbreak Associated with Chicken Liver Pâté Using PFGE and WGS
| Autor: | Joakim Ågren, M. Löfdahl, E. Olsson Engvall, Elina Lahti, Ingrid Hansson |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genotype Epidemiology 030106 microbiology Campylobacteriosis Biology medicine.disease_cause Disease Outbreaks Microbiology Foodborne Diseases 03 medical and health sciences 0302 clinical medicine Campylobacter Infections Pulsed-field gel electrophoresis medicine Animals Humans Food microbiology 030212 general & internal medicine General Veterinary General Immunology and Microbiology Campylobacter Public Health Environmental and Occupational Health Outbreak medicine.disease Electrophoresis Gel Pulsed-Field Infectious Diseases Liver Food Microbiology Multilocus sequence typing Flock Chickens |
| Zdroj: | Zoonoses and Public Health. 64:14-20 |
| ISSN: | 1863-1959 |
| DOI: | 10.1111/zph.12272 |
| Popis: | In May 2012, an outbreak of campylobacteriosis occurred in southern Sweden at a wedding reception affecting 44 persons. A total of 17 cases were notified (13 were culture positive for Campylobacter spp.). Epidemiological investigation suspected chicken liver pâté as the source of infection. The liver pâté had been deliberately undercooked, lightly fried to keep the right texture and mixed with spices. Campylobacter isolates from six cases as well as three Campylobacter isolates from chicken flocks previously raised by the producer delivering the liver were subtyped using pulsed-field gel electrophoresis and whole-genome sequencing. Indistinguishable PFGE profiles were identified among five human and one chicken C. jejuni isolates as well among the two C. coli isolates, one from a human case and one from a chicken. WGS supported the PFGE findings; the six C. jejuni isolates belonged to one cluster. All these six isolates were of MLST type ST 50 (ST-CC 21). This study highlights the importance of a combination of strict biosecurity at the flock-level as well as adequate cooking of chicken liver to prevent transmission of Campylobacter to humans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text