Low-density lipoprotein receptor and apolipoprotein A-I and B expression in human enterocytes
| Autor: | H. Bryan Brewer, Thomas L. Eggerman, Irina G. Safonova, D D Sviridov, Jeffrey M. Hoeg, Stephen J. Demosky |
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| Rok vydání: | 2002 |
| Předmět: |
Cell type
Apolipoprotein B Liver cytology Enterocyte Biology Intestinal mucosa Intestine Small medicine Tumor Cells Cultured Humans RNA Messenger Intestinal Mucosa Receptor Apolipoproteins B Apolipoprotein A-I Gastroenterology nutritional and metabolic diseases Blotting Northern Molecular biology medicine.anatomical_structure Enterocytes Biochemistry Gene Expression Regulation Liver Receptors LDL LDL receptor biology.protein lipids (amino acids peptides and proteins) Electrophoresis Polyacrylamide Gel Lipoprotein |
| Zdroj: | Digestion. 67(1-2) |
| ISSN: | 0012-2823 |
| Popis: | Low-density lipoprotein receptor (LDL-R) was found to be expressed in human small intestine epithelial cells, enterocytes. The relative abundance of LDL-R mRNA and protein was compared with that of apolipoproteins A-I (apoA-I) and B (apoB) in enterocytes and two other cell types: CaCo-2 and HepG2. The LDL-R mRNA content was comparable in three cell types. Human enterocytes expressed 5.2- to 14-fold more apoA-I mRNA than the other cells. In contrast, HepG2 cells expressed 10-to 19-fold more apoB mRNA than CaCo-2 cells and human enterocytes. Immunoprecipitation of [35S]methionine pulse-labeled intracellular proteins from these cell types demonstrated that human enterocytes synthesize more apoA-I and apoB, while HepG2 cells synthesize a slightly higher amount of LDL-R. |
| Databáze: | OpenAIRE |
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