Glutamine protects both transcellular and paracellular pathways of chick intestinal calcium absorption under oxidant conditions
| Autor: | Nori Tolosa de Talamoni, Gabriela Díaz de Barboza, Adriana Pérez, Luciana Moine, Cristina Alicia Maldonado |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Physiology Duodenum Glutamine Inflammation Protective Agents digestive system Biochemistry Avian Proteins 03 medical and health sciences 0302 clinical medicine Gene expression medicine Autophagy Animals Transcellular Molecular Biology Calcium metabolism urogenital system Chemistry Vitamin K 3 Transporter Oxidants Ruthenium Red Cell biology 030104 developmental biology Gene Expression Regulation Intestinal Absorption 030220 oncology & carcinogenesis Paracellular transport Calcium medicine.symptom tissues Chickens Signal Transduction |
| Zdroj: | Comparative biochemistry and physiology. Part A, Molecularintegrative physiology. 238 |
| ISSN: | 1531-4332 |
| Popis: | Glutamine (GLN) avoids the inhibition of the intestinal Ca2+ absorption caused by menadione (MEN) through oxidative stress. The purpose of this study was to elucidate whether molecules of transcellular and/or paracellular pathways of intestinal Ca2+ absorption are involved in the GLN action and underlying mechanisms. One-month old chicks were divided in four groups: 1) controls, 2) MEN treated, 3) GLN treated and 4) GLN + MEN treated. The morphology of intestinal villi, the intestinal Ca2+ absorption and the molecules involved in the transcellular and paracellular pathways were analyzed. Markers of autophagy and inflammation were also evaluated. The data demonstrated that GLN protected both transcellular and paracellular pathways. GLN avoided morphological changes in the intestine caused by MEN. GLN protected the gene expression of transporters involved in the transcellular pathway and the gene and protein expression of molecules belonging to the paracellular pathways altered by MEN. GLN increased the LC3-II protein expression and the number of acidic vesicular organelles, markers of autophagy, and blocked an increase in the NFkB protein expression in the nuclei and in the IL-6 gene expression caused by MEN. In conclusion, GLN protects both transcellular and paracellular pathways of intestinal Ca2+ absorption by increasing autophagy and blocking inflammation. |
| Databáze: | OpenAIRE |
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