The inhibition of dentifrice containing the lotus leaf-derived inhibitor on periodontitis-related bacteria in vitro
| Autor: | Zhu-ting Xu, Ming-yu Li |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Toothpaste
business.product_category biology Chemistry fungi food and beverages biology.organism_classification Antimicrobial Sensitivity and Specificity Microbiology Plant Leaves Anti-Infective Agents Actinobacillus Dentifrice Actinomyces naeslundii Lotus Lotus effect Food science Fusobacterium nucleatum business Periodontitis General Dentistry Bacteria Dentifrices Phytotherapy |
| Zdroj: | International dental journal. 57(5) |
| ISSN: | 0020-6539 |
| Popis: | Objectives: To search for a substance in lotus leaf that could efficiently be used in toothpaste for preventing periodontal disease. Methods: Dried lotus leaves were hydrated and fractionated using 40% butanol and 40% ethanol extraction. The extracts and the residue were evaluated by antimicrobial sensitivity tests of broth micro-dilution methods on 96-microwell plates for inhibition of five species of oral bacteria: Actinobacillus actinomycetemcomitans Y4, Actinomyces viscosus 19246, Porphyromonas gingivalis 33277, Fusobacterium nucleatum 25586, and Actinomyces naeslundii wvl 45. The most active antimicrobial extract was then ready to use in toothpaste. A water extract of the toothpaste was prepared and its inhibitory activity tested. The potency of the lotus leaf extracts was compared to inhibition observed by three commercially available toothpastes. Results: The butanol-extracted material was separated into four fractions on silica gel. Fraction #3, the fraction containing the most potent inhibitor, was formulated into toothpaste. The toothpaste containing the extract showed an inhibition effect on suspicious peridontitis-causing bacteria. Conclusion: An extract from the lotus leaf is effective in inhibiting peridontitis-causing bacteria. The toothpaste containing the lotus leaf-derived inhibitor could be useful in preventing periodontal disease. |
| Databáze: | OpenAIRE |
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