Effect of particle size and dispersion status on cytotoxicity and genotoxicity of zinc oxide in human bronchial epithelial cells
| Autor: | Minnamari Vippola, Maciej Stępnik, Hanna K. Lindberg, Hilkka Järventaus, Julia Catalán, Joanna Roszak, Satu Suhonen, Hannu Norppa |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Health
Toxicology and Mutagenesis ta221 Metal Nanoparticles Nanoparticle chemistry.chemical_element Bronchi 02 engineering and technology Zinc 010501 environmental sciences ta3111 medicine.disease_cause 01 natural sciences Cell Line Toxicology Genetics medicine Animals Humans micronucleus Particle Size Bovine serum albumin Cytotoxicity 0105 earth and related environmental sciences Micronucleus Tests biology Chemistry ta1184 nanoparticle genotoxicity ta1182 zinc oxide Epithelial Cells Serum Albumin Bovine ta3142 021001 nanoscience & nanotechnology Comet assay Micronucleus test biology.protein DNA damage Cattle Comet Assay Particle size 0210 nano-technology Genotoxicity Nuclear chemistry |
| Zdroj: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 805:7-18 |
| ISSN: | 1383-5718 |
| DOI: | 10.1016/j.mrgentox.2016.05.008 |
| Popis: | Data available on the genotoxicity of zinc oxide (ZnO) nanoparticles (NPs) are controversial. Here, we examined the effects of particle size and dispersion status on the cytotoxicity and genotoxicity of nanosized and fine ZnO, in the presence and absence of bovine serum albumin (BSA; 0.06%) in human bronchial epithelial BEAS-2B cells. Dynamic light scattering analysis showed the most homogenous dispersions in water alone for nanosized ZnO and in water with BSA for fine ZnO. After a 48-h treatment, both types of ZnO were cytotoxic within a similar, narrow dose range (1.5-3.0μg/cm(2)) and induced micronuclei at a near toxic dose range (1.25-1.75μg/cm(2)), both with and without BSA. In the comet assay, nanosized ZnO (1.25-1.5μg/cm(2)), in the absence of BSA, caused a statistically significant increase in DNA damage after 3-h and 6-h treatments, while fine ZnO did not. Our findings may be explained by better uptake or faster intracellular dissolution of nanosized ZnO without BSA during short treatments (3-6h; the comet assay), with less differences between the two ZnO forms after longer treatments (>48h; the in vitro micronucleus test). As ZnO is genotoxic within a narrow dose range partly overlapping with cytotoxic doses, small experimental differences e.g. in the dispersion of ZnO particles may have a substantial effect on the genotoxicity of the nominal doses added to the cell culture. |
| Databáze: | OpenAIRE |
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