Ultrasound-Guided Minimally Invasive Tissue Sampling: A Minimally Invasive Autopsy Strategy During the COVID-19 Pandemic in Brazil, 2020
| Autor: | Amaro Nunes Duarte-Neto, Luiz Fernando Ferraz da Silva, Renata Aparecida de Almeida Monteiro, Jair Theodoro Filho, Thabata Larissa Luciano Ferreira Leite, Catia Sales de Moura, Michele Soares Gomes-Gouvêa, João Renato Rebellho Pinho, Cristina Takami Kanamura, Ellen Pierre de Oliveria, Kely Cristina Soares Bispo, Cássia Arruda, Aline Brito dos Santos, Flavia Cristina Gonçalves Aquino, Elia Garcia Caldini, Thais Mauad, Paulo Hilário Nascimento Saldiva, Marisa Dolhnikoff |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
ultrasound guided biopsy minimally invasive tissue sampling SARS-CoV-2 COVID-19 Supplement Articles autopsy safety Infectious Diseases AcademicSubjects/MED00290 COVID-19 Testing Humans pathology Autopsy minimally invasive autopsy Pandemics Brazil Ultrasonography Interventional |
| Zdroj: | Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America |
| ISSN: | 1537-6591 1058-4838 |
| Popis: | Background Minimally invasive autopsies, also known as minimally invasive tissue sampling (MITS), have proven to be an alternative to complete diagnostic autopsies (CDAs) in places or situations where this procedure cannot be performed. During the coronavirus disease 2019 (COVID-19) pandemic, CDAs were suspended by March 2020 in Brazil to reduce biohazard. To contribute to the understanding of COVID-19 pathology, we have conducted ultrasound (US)–guided MITS as a strategy. Methods This case series study includes 80 autopsies performed in patients with COVID-19 confirmed by laboratorial tests. Different organs were sampled using a standardized MITS protocol. Tissues were submitted to histopathological analysis as well as immunohistochemical and molecular analysis and electron microscopy in selected cases. Results US-guided MITS proved to be a safe and highly accurate procedure; none of the personnel were infected, and accuracy ranged from 69.1% for kidney, up to 90.1% for lungs, and reaching 98.7% and 97.5% for liver and heart, respectively. US-guided MITS provided a systemic view of the disease, describing the most common pathological findings and identifying viral and other infectious agents using ancillary techniques, and also allowed COVID-19 diagnosis confirmation in 5% of the cases that were negative in premortem and postmortem nasopharyngeal/oropharyngeal swab real-time reverse-transcription polymerase chain reaction. Conclusions Our data showed that US-guided MITS has the capacity similar to CDA not only to identify but also to characterize emergent diseases. |
| Databáze: | OpenAIRE |
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