Limited proteolysis of tumor cells increases their plasmin-binding ability
| Autor: | Mercedes Camacho, Pierre Burtin, Marie-Claude Fondaneche |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Plasmin
Proteolysis Lysine Biophysics Tumor cells Carboxypeptidases Biochemistry Aprotinin Structural Biology Tumor Cells Cultured Genetics medicine Humans Trypsin Fibrinolysin Binding site Molecular Biology biology medicine.diagnostic_test Chemistry Fibrinogen Cell Biology Carboxypeptidase B (Tumor cell) Binding ability Lysine-binding site Colonic Neoplasms biology.protein Electrophoresis Polyacrylamide Gel Peptide Hydrolases medicine.drug circulatory and respiratory physiology |
| Zdroj: | FEBS Letters. (1-2):21-24 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(89)80183-8 |
| Popis: | Mild proteolytic treatment of SW 1116 tumor cells with trypsin or plasmin increases their plasmin-binding ability considerably by increasing the number of binding sites without altering their affinity. This mechanism may be operative for increasing the concentration of active plasmin at the surface of tumor cells. C-terminal lysine residues are involved in plasmin binding to cells, since treatment of cells with carboxypeptidase B decreases this binding by 50%. |
| Databáze: | OpenAIRE |
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