Metabolism of testosterone sulfate in the rat: Analysis of biliary metabolites
| Autor: | Hakozaki Misako, Kinuyama Yuko, Matsui Michio |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Metabolite Clinical Biochemistry Intraperitoneal injection Glucuronates Biology Androsterone Tritium Biochemistry chemistry.chemical_compound Sex Factors Endocrinology Column chromatography Internal medicine medicine Animals Bile Electrophoresis Paper Testosterone Carbon Radioisotopes Molecular Biology Pharmacology Testosterone Sulfate Organic Chemistry Metabolism Sulfuric Acids Androstane-3 17-diol Rats Aglycone chemistry Female |
| Zdroj: | Steroids. 25:637-648 |
| ISSN: | 0039-128X |
| DOI: | 10.1016/0039-128x(75)90018-5 |
| Popis: | Following intraperitoneal injection of a mixture of testosterone-7-3-H-17-sulfate and testosterone-4-14-C into male and female rats with bile fistulas, biliary metabolites were separated and purified by a combination of column chromatography, enzymic hydrolysis or solvolysis of the conjugate fractions and identification of the liberated aglycones. The injected steroids were extensively metabolized and excreted predominantly in the bile. The major portion of the 3H was excreted in the disulfate fraction in both sexes. Solvolysis of the disulfate revealed the sex-specific aglycone pattern: 5alpha-Androstane-3beta,17beta-diol was the major metabolite in the male rat, whereas 5alpha-androstane-3alpha,17beta-diol and polar steroids were found in the female. In marked contrast, testosterone was metabolized in a different way than testosterone sulfate. 14-C radioactivity was distributed in monoglucosiduronate, monosulfate, and diconjugate fractions. Analysis of the aglycones showed that polar steroids were the main metabolites in the male. In the female, testosterone was metabolized to polar steroids, androsterone, and 5alpha-androstane-3alpha,17beta-diol. |
| Databáze: | OpenAIRE |
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