Calcium channel-dependent induction of long-term synaptic plasticity at excitatory Golgi cell synapses of cerebellum
| Autor: | Laura Botta, Ileana Montagna, Francesca Prestori, Simona Tritto, Francesca Locatelli, Egidio D'Angelo, Teresa Soda |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Mossy fiber (hippocampus) Cerebellum Calcium Channels L-Type Cerebellar Golgi Cells Long-Term Potentiation Receptors N-Methyl-D-Aspartate Synapse Mice 03 medical and health sciences Nerve Fibers 0302 clinical medicine Calcium imaging Golgi cell medicine Animals Research Articles 030304 developmental biology 0303 health sciences Chemistry General Neuroscience Excitatory Postsynaptic Potentials Long-term potentiation 030104 developmental biology medicine.anatomical_structure Synapses Synaptic plasticity Excitatory postsynaptic potential NMDA receptor Female Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | The Journal of Neuroscience, Vol. 41, No. 15 J Neurosci |
| DOI: | 10.1101/2019.12.19.882944 |
| Popis: | The Golgi cells, together with granule cells and mossy fibers, form a neuronal microcircuit regulating information transfer at the cerebellum input stage. Despite theoretical predictions, little was known about long-term synaptic plasticity at Golgi cell synapses. Here we have used whole-cell patch-clamp recordings and calcium imaging to investigate long-term synaptic plasticity at excitatory synapses impinging on Golgi cells. In acute mouse cerebellar slices, mossy fiber theta-burst stimulation (TBS) could induce either long-term potentiation (LTP) or long-term depression (LTD) at mossy fiber-Golgi cell and granule cell-Golgi cell synapses. This synaptic plasticity showed a peculiar voltage-dependence, with LTD or LTP being favored when TBS induction occurred at depolarized or hyperpolarized potentials, respectively. LTP required, in addition to NMDA channels, activation of T-type Ca2+ channels, while LTD required uniquely activation of L-type Ca2+ channels. Notably, the voltage-dependence of plasticity at the mossy fiber-Golgi cell synapses was inverted with respect to pure NMDA receptor-dependent plasticity at the neighboring mossy fiber-granule cell synapse, implying that the mossy fiber presynaptic terminal can activate different induction mechanisms depending on the target cell. In aggregate, this result shows that Golgi cells show cell-specific forms of long-term plasticity at their excitatory synapses, that could play a crucial role in sculpting the response patterns of the cerebellar granular layer.Significance statementThis paper shows for the first time a novel form of Ca2+ channel-dependent synaptic plasticity at the excitatory synapses impinging on cerebellar Golgi cells. This plasticity is bidirectional and inverted with respect to NMDA receptor-dependent paradigms, with LTD and LTP being favored at depolarized and hyperpolarized potentials, respectively. Furthermore, LTP and LTD induction requires differential involvement of T-ype and L-type voltage-gated Ca2+channels rather than the NMDA receptors alone. These results, along with recent computational predictions, support the idea that Golgi cell plasticity could play a crucial role in controlling information flow through the granular layer along with cerebellar learning and memory. |
| Databáze: | OpenAIRE |
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