Photoactivated capture molecule immobilization in plasmonic nanoapertures in the ultraviolet
| Autor: | Mohit Diwekar, Steve Blair, Sachin Attavar |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Materials science Oligonucleotide business.industry Biomolecule Biomedical Engineering Bioengineering Nanotechnology Near and far field General Chemistry medicine.disease_cause Biochemistry Fluorescence Coupling (electronics) chemistry Biotinylation medicine Optoelectronics business Ultraviolet Plasmon |
| Zdroj: | Lab on a Chip. 11:841 |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/c0lc00498g |
| Popis: | We demonstrate a photoactivated surface coupling scheme for achieving spatial overlap between biomolecules of interest and optical near field excitation. Using aluminium nanoapertures, we obtained increased coupling efficiency of biotinylated capture probe oligos to the photoactivated surface due to ~3× nanoaperture enhancement of UV light. We further validate DNA sensor functionality via the hybridization of Cy-5 labeled target oligos, with up to 8× fluorescence enhancement obtained from a commercial microarray scanner. This generic photoimmobilization strategy is an essential step to realizing miniaturized plasmon enhanced detection arrays by virtue of localizing capture molecules to the region of plasmonic enhancement. |
| Databáze: | OpenAIRE |
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