Caracterisation et evolution physiologique des recepteurs pour les 'insulin-like growth factors' I et II (IGFs) dans la glande mammaire de brebis
| Autor: | Catherine Disenhaus, Lucette Belair, Jean Djiane |
|---|---|
| Přispěvatelé: | ProdInra, Migration, Institut francilien recherche, innovation et société (IFRIS), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.)-Institut National de la Recherche Agronomique (INRA)-École des hautes études en sciences sociales (EHESS)-OST-Université Paris-Est Marne-la-Vallée (UPEM)-ESIEE Paris-Centre National de la Recherche Scientifique (CNRS), Laboratoire de physiologie de la lactation, Institut National de la Recherche Agronomique (INRA), Revues Inra, Import, Institut National de la Recherche Agronomique (INRA)-École des hautes études en sciences sociales (EHESS)-OST-Université Paris-Est Marne-la-Vallée (UPEM)-Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.)-ESIEE Paris-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | francouzština |
| Rok vydání: | 1988 |
| Předmět: |
endocrine system
Embryology medicine.medical_specialty medicine.medical_treatment Cellular differentiation [SDV]Life Sciences [q-bio] Mammary gland Medicine (miscellaneous) Internal medicine Lactation [SDV.BDD] Life Sciences [q-bio]/Development Biology medicine Receptor ED50 ComputingMilieux_MISCELLANEOUS [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology Relaxin Chemistry Insulin Prolactin [SDV] Life Sciences [q-bio] [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition medicine.anatomical_structure Endocrinology Reproductive Medicine PHYSIOLOGIE Animal Science and Zoology hormones hormone substitutes and hormone antagonists Developmental Biology Food Science |
| Zdroj: | Reproduction Nutrition Development Reproduction Nutrition Development, EDP Sciences, 1988, 28 (2A), pp.241-252 |
| ISSN: | 0926-5287 1297-9708 |
| Popis: | Membrane preparations were obtained by differential centrifugations of ewe mammary gland homogenates. These membrane preparations contained specific receptors for IGF1 and IGF2 which possess high affinities for their specific ligands (Ka .5 to 1.5 10(9) M-1). Maximum binding of 125I IGF1 was obtained after 48 h at 4 degrees C. This binding was inhibited by unlabelled IGF1 (ED50 = 14 ng/ml), partially inhibited by high concentrations of insulin (50 micrograms/ml). Prolactin (oPRL), growth hormone (bGH) or relaxin (Rel) were without effect. Maximum binding of 125I IGF2 was obtained after 6 h at 20 degrees C. This binding was inhibited by unlabelled IGF2 (ED50 = 44 ng/ml), partially inhibited by IGF1 (ED50 = 200 ng/ml) and unmodified by INS, PRL, bGH or Rel. Receptor numbers for IGF1 were significantly higher (p less than 0.01) on day 100 of pregnancy (N = 480 +/- 17 fmoles/mg proteins) compared to day 20 of lactation (N = 174 +/- 21 fmoles/mg). The numbers of IGF2 receptors were always higher than those of IGF1 receptors. During pregnancy the numbers of IGF2 receptors (N = 1,860 +/- 157 fmoles/mg) were also higher than during lactation. These results suggest that the mammary gland may constitute a target organ for IGFs. These factors could be involved in the regulation of mammary gland development and during cell differentiation. |
| Databáze: | OpenAIRE |
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