Co-localization and Distribution of Cerebral APP and SP1 and its Relationship to Amyloidogenesis
| Autor: | Amy Anderson, G. Jean Harry, Riyaz Basha, Deborah C. Rice, Debomoy K. Lahiri, Bryan Maloney, Brian Brock, Nasser H. Zawia, Katie DiPalma |
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| Rok vydání: | 2008 |
| Předmět: |
Cerebellum
Sp1 Transcription Factor Hippocampus Article Amyloid beta-Protein Precursor Pregnancy mental disorders medicine Amyloid precursor protein Animals Rats Long-Evans Protein precursor Transcription factor Cellular localization Neocortex biology Chemistry General Neuroscience Amyloidosis General Medicine Immunohistochemistry Rats Cell biology Macaca fascicularis Psychiatry and Mental health Clinical Psychology medicine.anatomical_structure biology.protein Female Geriatrics and Gerontology Pyramidal cell Biogenesis |
| Zdroj: | Journal of Alzheimer's Disease. 13:71-80 |
| ISSN: | 1875-8908 1387-2877 |
| Popis: | Alzheimer's disease is characterized by amyloid-beta peptide (Abeta)-loaded plaques in the brain. Abeta is a cleavage fragment of amyloid-beta protein precursor (APP) and over production of APP may lead to amyloidogenesis. The regulatory region of the APP gene contains consensus sites recognized by the transcription factor, specificity protein 1 (SP1), which has been shown to be required for the regulation of APP and Abeta. To understand the role of SP1 in APP biogenesis, herein we have characterized the relative distribution and localization of SP1, APP, and Abeta in various brain regions of rodent and primate models using immunohistochemistry. We observed that overall distribution and cellular localization of SP1, APP, and Abeta are similar and neuronal in origin. Their distribution is abundant in various layers of neocortex, but restricted to the Purkinje cell layer of the cerebellum, and the pyramidal cell layer of hippocampus. These findings suggest that overproduction of Abeta in vivo may be associated with transcriptional pathways involving SP1 and the APP gene. |
| Databáze: | OpenAIRE |
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