Hormonal Regulation and Crosstalk of Auxin/Cytokinin Signaling Pathways in Potatoes In Vitro and in Relation to Vegetation or Tuberization Stages

Autor: Georgy A. Romanov, Sergey N. Lomin, Svetlana V. Deigraf, Yulia A. Myakushina, Irina A. Getman, Igor V. Deyneko, Oksana O. Kolachevskaya
Rok vydání: 2021
Předmět:
0106 biological sciences
0301 basic medicine
Sucrose
Cytokinins
01 natural sciences
phytohormone signaling
chemistry.chemical_compound
Gene expression
heterocyclic compounds
Biology (General)
Spectroscopy
chemistry.chemical_classification
promoter cis-elements
food and beverages
General Medicine
Phenotype
Computer Science Applications
Cell biology
Chemistry
Crosstalk (biology)
Cytokinin
Signal transduction
Signal Transduction
QH301-705.5
Plant Development
Biology
signaling crosstalk
Genes
Plant
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
cytokinin
Auxin
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
Gene
sugar effects
Solanum tuberosum
Indoleacetic Acids
Organic Chemistry
fungi
gene clustering
030104 developmental biology
chemistry
gene expression
auxin
010606 plant biology & botany
Hormone
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 15
International Journal of Molecular Sciences, Vol 22, Iss 8207, p 8207 (2021)
ISSN: 1422-0067
Popis: Auxins and cytokinins create versatile regulatory network controlling virtually all aspects of plant growth and development. These hormonal systems act in close contact, synergistically or antagonistically, determining plant phenotype, resistance and productivity. However, the current knowledge about molecular interactions of these systems is still scarce. Our study with potato plants aimed at deciphering potential interactions between auxin and cytokinin signaling pathways at the level of respective gene expression. Potato plants grown on sterile medium with 1.5% (vegetation) or 5% (tuberization) sucrose were treated for 1 h with auxin or cytokinin. Effects of these two hormones on expression profiles of genes belonging to main signaling pathways of auxin and cytokinin were quantified by RT-qPCR. As a result, several signaling genes were found to respond to auxin and/or cytokinin by up- or down-regulation. The observed effects were largely organ-specific and depended on sucrose content. Auxin strongly reduced cytokinin perception apparatus while reciprocal cytokinin effect was ambiguous and sucrose-dependent. In many cases, functional clustering of genes of the same family was observed. Promoters in some clusters are enriched with canonic hormone-response cis-elements supporting their direct sensitivity to hormones. Collectively, our data shed new light on the crosstalk between auxin- and cytokinin signaling pathways.
Databáze: OpenAIRE
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