Prostaglandins alter methacholine-induced secretion in ferret in vitro trachea
| Autor: | M. E. Deffebach, Hilmi Islami, A. M. Price, J. G. Widdicombe, S. E. Webber |
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| Rok vydání: | 1990 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Physiology Serum albumin In Vitro Techniques Dinoprost chemistry.chemical_compound Physiology (medical) Internal medicine medicine Animals Methacholine Compounds Secretion Bovine serum albumin Alprostadil Methacholine Chloride biology Chemistry Prostaglandin D2 Albumin Ferrets Muscle Smooth Serum Albumin Bovine Cell Biology respiratory system In vitro Trachea Kinetics Endocrinology biology.protein Prostaglandins Cholinergic lipids (amino acids peptides and proteins) Methacholine Muramidase Lysozyme medicine.drug |
| Zdroj: | The American journal of physiology. 258(2 Pt 1) |
| ISSN: | 0002-9513 |
| Popis: | The ferret trachea was mounted in an organ bath containing Krebs-Henseleit solution with additional bovine serum albumin (BSA). Tracheal secretions were collected and analyzed for albumin, and lysozyme, a specific marker of serous cell secretion. The total secretion volume and output and concentrations of albumin and lysozyme were calculated. Secretion was stimulated with methacholine (20 microM) (Mch), and the effects of the prostaglandins PGD2, PGE1, and PGF2 alpha on methacholine-induced secretion were studied. All responses were dose dependent. PGF2 alpha at 10(-5) M increased the volume of Mch-stimulated secretion twofold, the lysozyme output sixfold, and concentration over threefold, while decreasing the albumin transport by one-half. PGD2 at 10(-5) M reduced Mch-induced secretion volume to 75% control, increased albumin transport to 135%, without affecting lysozyme secretion. PGE1 at 10(-5) M increased Mch-stimulated albumin transport and concentration over twofold, decreased lysozyme release to less than one-third of control, and had no effect on secretion volume. PGE1 caused the albumin concentration to exceed that of the outer bath, indicating active transport. We conclude that prostaglandins selectively alter tracheal secretion induced by cholinergic stimuli. |
| Databáze: | OpenAIRE |
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