Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows
| Autor: | S. De Campeneere, Eric Cox, Vanessa Meganck, Bruno Goddeeris, Sofie Piepers, Geert Opsomer, M. Van Eetvelde, Miel Hostens |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
NEONATAL CALVES
IGG CONCENTRATION medicine.medical_specialty Cellular immunity leukocytes medicine.medical_treatment NEWBORN CALVES MITOGENIC RESPONSES BOVINE POLYMORPHONUCLEAR LEUKOCYTES Passive immunity PERIPARTURIENT PERIOD Biology Fatty Acids Nonesterified PERIPHERAL-BLOOD Peripheral blood mononuclear cell Flow cytometry Pregnancy Internal medicine negative energy balance Genetics medicine Ingestion Animals dairy cows Veterinary Sciences MILK T-LYMPHOCYTES Cluster of differentiation medicine.diagnostic_test 3-Hydroxybutyric Acid Colostrum NEUTROPHIL FUNCTION Parity Endocrinology Cross-Sectional Studies colostrum biology.protein Body Composition Leukocytes Mononuclear Animal Science and Zoology Cattle Female Antibody MAMMARY-GLAND SECRETIONS Food Science |
| Zdroj: | JOURNAL OF DAIRY SCIENCE |
| ISSN: | 0022-0302 |
| Popis: | In neonatal calves, the ingestion of colostrum is imperative for preventing infectious diseases. Investigations into the transfer of passive immunity of cattle have primarily focused on the importance of colostral immunoglobulins, with a recent increase in focus on understanding the role of colostral leukocytes. The main objective of the present study was to measure the influence of parity, body condition score, serum nonesterified fatty acids, and serum beta-hydroxybutyrate concentrations of periparturient cows on phenotype and mitogen-and antigen-induced proliferative capacity of bovine colostral leukocytes. Holstein-Friesian cows (n = 141) were intramuscularly vaccinated at 60 and 30 d before the expected parturition date with a tetanus toxoid vaccine. Of these 141 animals, 28 primiparous and 72 multiparous cows were sampled immediately. after parturition. Colostrum mononuclear cell populations were identified by flow cytometry using bovine cluster of differentiation markers, and the proliferative capacity of these cells was determined using a H-3-thymidine proliferation assay. Under-conditioned cows had a significantly higher percentage of colostral macrophages than normal-conditioned animals, whereas over-conditioned cows had significantly more colostral B-lymphocytes. Serum beta-hydroxybutyrate was significantly associated with higher numbers of colostral T-lymphocytes and macrophages. Heifers had significantly higher mitogen- and antigen-induced proliferation of their colostral leukocytes than third parity or older cows. In conclusion, body condition score, parity, and serum beta-hydroxybutyrate concentration of periparturient high-yielding dairy cows were shown to influence the number of colostral macrophages or the mitogen-and antigen-induced proliferation of colostral leukocytes, possibly influencing the cellular immunity of the newborn calf. |
| Databáze: | OpenAIRE |
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