Identification of a common conserved neutralizing linear B-cell epitope in the VP3 protein of waterfowl parvoviruses
| Autor: | Nana Sui, Jingjing Lan, Yupeng Yang, Shijin Jiang, Zhijing Xie, Hanqing Li, Caiyu Lian, Ruihua Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
040301 veterinary sciences
medicine.drug_class animal diseases viruses Monoclonal antibody Epitope Virus 0403 veterinary science Parvoviridae Infections Viral Proteins Food Animals Parvovirinae Geese Waterfowl medicine Animals B cell Epizootic Poultry Diseases Goose parvovirus General Immunology and Microbiology biology 0402 animal and dairy science virus diseases Antibodies Monoclonal 04 agricultural and veterinary sciences biology.organism_classification medicine.disease 040201 dairy & animal science Virology medicine.anatomical_structure Epitopes B-Lymphocyte Animal Science and Zoology Muscovy duck parvovirus |
| Zdroj: | Avian pathology : journal of the W.V.P.A. 49(4) |
| ISSN: | 1465-3338 |
| Popis: | Waterfowl parvoviruses (WPVs) including goose parvovirus (GPV), novel GPV-related virus (NGPV) and Muscovy duck parvovirus (MDPV) cause significant economic losses and epizootic threat to the waterfowl industries, and little is known about the B-cell epitopes of WPVs. In this study, a monoclonal antibody (mAb) 5B5 against the VP3 protein of NGPV was used to identify the possible epitope in the three kinds of WPVs. The mAb 5B5 had neutralizing activities to the three viruses, and reacted with the conserved linear B-cell epitopes of 438LHNPPP443 in VP3 protein of GPV, NGPV and MDPV. To the authors' best knowledge, this is the first report on identification of the common conserved neutralizing linear B-cell epitope on VP3 protein of three different WPVs, which would facilitate the development of a novel immunodiagnostic assay for rapid detection of WPV infection. |
| Databáze: | OpenAIRE |
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