Zika Virus Fatally Infects Wild Type Neonatal Mice and Replicates in Central Nervous System
| Autor: | Shuxuan Li, Chunlian Zhong, Chunye Chen, Huan Zhao, Najealicka Armstrong, Qiyi Tang, Wan Junkai, Wei Wang, Che Liu, Wangheng Hou, Tong Cheng, Hua Zhu, Jian Liu, Ningshao Xia |
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| Rok vydání: | 2018 |
| Předmět: |
Central Nervous System
Male 0301 basic medicine Microcephaly Viral pathogenesis lcsh:QR1-502 Virus Replication Hippocampus lcsh:Microbiology Zika virus Pathogenesis Mice Testis Paralysis microcephaly Cerebral Cortex Mice Inbred BALB C Mice Inbred ICR biology Zika Virus Infection Transmission (medicine) pathogenesis Brain Olfactory Bulb 3. Good health Flavivirus Infectious Diseases medicine.anatomical_structure Liver medicine.symptom Immunocompetence Central nervous system Article Zika virus (ZIKV) 03 medical and health sciences Virology medicine Animals Zika Virus medicine.disease biology.organism_classification Mice Inbred C57BL Disease Models Animal 030104 developmental biology Animals Newborn neonatal mouse |
| Zdroj: | Viruses Viruses; Volume 10; Issue 1; Pages: 49 Viruses, Vol 10, Iss 1, p 49 (2018) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v10010049 |
| Popis: | Zika virus (ZIKV) has been defined as a teratogenic pathogen behind the increased number of cases of microcephaly in French Polynesia, Brazil, Puerto Rico, and other South American countries. Experimental studies using animal models have achieved tremendous insight into understanding the viral pathogenesis, transmission, teratogenic mechanisms, and virus–host interactions. However, the animals used in published investigations are mostly interferon (IFN)-compromised, either genetically or via antibody treatment. Herein, we studied ZIKV infection in IFN-competent mice using African (MR766) and Asian strains (PRVABC59 and SZ-WIV01). After testing four different species of mice, we found that BALB/c neonatal mice were resistant to ZIKV infection, that Kunming, ICR and C57BL/6 neonatal mice were fatally susceptible to ZIKV infection, and that the fatality of C57BL/6 neonates from 1 to 3 days old were in a viral dose-dependent manner. The size and weight of the brain were significantly reduced, and the ZIKV-infected mice showed neuronal symptoms such as hind-limb paralysis, tremor, and poor balance during walking. Pathologic and immunofluorescent experiments revealed that ZIKV infected different areas of the central nervous system (CNS) including gray matter, hippocampus, cerebral cortex, and spinal cord, but not olfactory bulb. Interestingly, ZIKV replicated in multiple organs and resulted in pathogenesis in liver and testis, implying that ZIKV infection may engender a high health risk in neonates by postnatal infection. In summary, we investigated ZIKV pathogenesis using an animal model that is not IFN-compromised. |
| Databáze: | OpenAIRE |
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