The recombinant plant Bauhinia bauhinioides elastase inhibitor reduces rat thrombus without alterations in hemostatic parameters
| Autor: | Daiane Hansen, Tatiana F. Ottaiano, Misako U. Sampaio, Maria Luiza Vilela Oliva, Mayara Vioto Valois, Antonio Miranda, Cleide de Oliveira, Francisco Humberto de Abreu Maffei |
|---|---|
| Přispěvatelé: | Universidade Federal de São Paulo (UNIFESP), Universidade Estadual Paulista (UNESP) |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Serine Proteinase Inhibitors Science medicine.medical_treatment Diseases Drug development 030204 cardiovascular system & hematology Pharmacology Biochemistry Article 03 medical and health sciences 0302 clinical medicine Bleeding time medicine Animals Humans Thrombus Rats Wistar Ligature Blood Coagulation Plant Proteins Prothrombin time Multidisciplinary medicine.diagnostic_test Pancreatic Elastase Chemistry Drug discovery Elastase Thrombosis Heparin medicine.disease Recombinant Proteins Rats Elastase inhibitor 030104 developmental biology Bauhinia Medicine Partial Thromboplastin Time medicine.drug Partial thromboplastin time circulatory and respiratory physiology |
| Zdroj: | Scientific Reports Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Made available in DSpace on 2022-04-29T08:30:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01 The anti-inflammatory effects of the plant protease inhibitor BbCI (Bauhinia bauhinioides cruzipain inhibitor), which blocks elastase, cathepsin G, and L, and proteinase 3 has been demonstrated. Here, we investigated the recombinant rBbCI-His(6) (containing a histidine tail) in an experimental venous thrombosis model of vena cava (VC) ligature in rats, comparing to heparin. We evaluate the effects of the inhibitors (native or recombinant) or heparin on the activated partial thromboplastin time (aPTT) and prothrombin time (PT) in human and rat plasmas. The rats undergoing treatment received a saline solution or increasing concentrations of rBbCI-His(6), heparin, or a mixture of both. After 4 h of ligature VC, thrombus, if present was removed and weighed. aPTT, PT, and cytokines were measured in blood collected by cardiac puncture. aPTT, PT, and bleeding time (BT) were also measured at the time of VC (vena cava) ligature. rBbCI-His(6) (0.45 or 1.40 mg/kg) does not alter aPTT, PT or BT. No differences in coagulation parameters were detected in rBbCI-His(6) treated rats at the time of VC ligature or when the thrombus was removed. There was a significant decrease in the weight of thrombus in the animals of the groups treated with the rBbCI-His(6) (1.40 mg/kg), with the rBbCI-His(6) mixture (1.40 mg/kg) + heparin (50 IU/kg) and heparin (100 IU/kg) in relation to control group (saline). The growth-related oncogene/keratinocyte chemoattractant (GRO/KC) serum levels in rats treated with rBbCI-His(6) (1.40 mg/kg) or heparin (200 IU/kg) were reduced. In the experimental model used, rBbCI-His(6) alone had an antithrombotic effect, not altering blood clotting or bleeding time. Departamento de Bioquímica Universidade Federal de São Paulo, Rua Três de Maio, 100 Departamento de Biofísica Universidade Federal de São Paulo Departamento de Cirurgia e Ortopedia Universidade Estadual Paulista Departamento de Cirurgia e Ortopedia Universidade Estadual Paulista |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|