Time-resolved metabolomics analysis of β-cells implicates the pentose phosphate pathway in the control of insulin release
| Autor: | Vladimir V. Sharoyko, Peter Spégel, Claes B. Wollheim, Cecilia Nagorny, Lotta Andersson, Geoffrey W. G. Sharp, Thomas Koeck, Siri Malmgren, Isabel Goehring, Anders P. H. Danielsson, Hindrik Mulder, Susanne G. Straub |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Pentose Phosphate Pathway/drug effects/physiology medicine.medical_specialty Islets of Langerhans/metabolism/secretion Metabolite medicine.medical_treatment Cell Respiration Pentose phosphate pathway Biology Biochemistry Pentose Phosphate Pathway Islets of Langerhans chemistry.chemical_compound Insulin-Secreting Cells Internal medicine Insulin Secretion Ribose medicine Insulin Metabolomics Animals Metabolomics/methods Rats Wistar ddc:612 Molecular Biology Cell Respiration/physiology Cells Cultured Insulin/secretion Secretory Pathway Secretory Pathway/drug effects Cell Biology Metabolism Glutathione Mitochondria/metabolism/physiology Glucose/pharmacology Mitochondria Rats Metabolic pathway Glucose Endocrinology chemistry Ribose 5-phosphate Insulin-Secreting Cells/chemistry/metabolism/secretion Metabolome |
| Zdroj: | Biochemical Journal, Vol. 450, No 3 (2013) pp. 595-605 |
| ISSN: | 0264-6021 |
| Popis: | Insulin secretion is coupled with changes in β-cell metabolism. To define this process, 195 putative metabolites, mitochondrial respiration, NADP+, NADPH and insulin secretion were measured within 15 min of stimulation of clonal INS-1 832/13 β-cells with glucose. Rapid responses in the major metabolic pathways of glucose occurred, involving several previously suggested metabolic coupling factors. The complexity of metabolite changes observed disagreed with the concept of one single metabolite controlling insulin secretion. The complex alterations in metabolite levels suggest that a coupling signal should reflect large parts of the β-cell metabolic response. This was fulfilled by the NADPH/NADP+ ratio, which was elevated (8-fold; P2.5-fold; P |
| Databáze: | OpenAIRE |
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