| Autor: |
Treinen, Chantal, Magosch, Olivia, Hoffmann, Mareen, Klausmann, Peter, W��rtz, Berit, Pfannstiel, Jens, Morabbi Heravi, Kambiz, Lilge, Lars, Hausmann, Rudolf, Henkel, Marius |
| Rok vydání: |
2021 |
| DOI: |
10.6084/m9.figshare.16907558.v1 |
| Popis: |
Additional file 1: Table S1. List of strains used for this study. Table S2. List of plasmids used for this study. Table S3. List of oligonucleotides used for this study according to Hoffmann et al. (2021). Table S4. Overview of the specificity test, employing various Bacillus sp.; (+) indicating ComX activity; (-) indicating that no ComX activity above the LOQ (42.7 MU) was determined. Table S5. Overview of process parameters of shake flask cultivations, including yield coefficients, highest surfactin titer Pmax [mg/L], highest biomass Xmax [g/L], highest ComX activity ComXmax [MU], as well as the ComX activity at the end of the cultivation ComX tend [MU]. Fig. S1. (a) Extracted ion chromatogram (XIC) from single ion monitoring (SIM) scan at m/z 681.8747 of cell-free supernatant from B. subtilis DSM 23778; (b) ESI-MS/MS spectrum of the precursor ion m/z 681.8747 at retention time 7.2 min (ComX isomer 1). Identity of the ComX pheromone was confirmed by b- and y-ion series as indicated in blue and red, respectively. Internal fragments are indicated in green. FA: farnesylation. The MS/MS spectrum of ComX isomer 2 at retention time 7.5 min showed almost identical fragment ions (data not shown). Fig. S2. Graphical illustration of ComX degradation studies. (a) Plotted are the measured ComX activity (gray bars) and the corresponding ComX concentration determined by mass spectrometry (black circles) over the cultivation time; (b) Plotted are the ComX activity (gray bars) and extracellular peptidase activity (black diamond) of heat-treated supernatant over the incubation time; (c) Plotted are the OD600 (black cross) of strain BKK31700, the corresponding ComX activity (gray bars) and extracellular peptidase activity (black diamond) over the cultivation time. Fig. S3. Time course of shake flask cultivations of B. subtilis DSM 10T employing 8, 20 and 40 g/L glucose. Plotted are the CDW (black cross), glucose (gray square) and ammonium (black triangle) depletion against the cultivation time in the upper part and ComX activity (white circle), surfactin concentration (gray circle) and extracellular peptidase activity (black diamond) against the cultivation time in the lower part of the figure. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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