GABAergic input to cholinergic nucleus basalis neurons
| Autor: | Michel Muhlethaler, Asaid Khateb, Barbara E. Jones, Mauro Serafin, Patrice Fort, S. Williams |
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| Přispěvatelé: | Centre médical universitaire de Genève (CMU), Département de Physiologie, Genève, Suisse, Centre médical universitaire, Centre de recherche en neurosciences de Lyon (CRNL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Team Physiopathologie des Réseaux Neuronaux Responsables du Cycle Veille-Sommeil, Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Montreal Neurological Institute and Hospital, McGill University = Université McGill [Montréal, Canada], Department of Neurology and Neurosurgery [Montreal], McGill University = Université McGill [Montréal, Canada]-McGill University = Université McGill [Montréal, Canada] |
| Rok vydání: | 1998 |
| Předmět: |
Baclofen
Substantia Innominata/physiology [SDV]Life Sciences [q-bio] Muscimol/pharmacology Synaptic Transmission 0302 clinical medicine Substantia Innominata Postsynaptic potential ComputingMilieux_MISCELLANEOUS gamma-Aminobutyric Acid 6-Cyano-7-nitroquinoxaline-2 3-dione Neurons 0303 health sciences Basal forebrain Glutamate Decarboxylase Muscimol General Neuroscience Neurons/drug effects/ physiology Immunohistochemistry Receptors GABA-B/agonists/antagonists & inhibitors/ physiology Prosencephalon/drug effects/ physiology GABAergic [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Bicuculline/pharmacology medicine.drug Guinea Pigs In Vitro Techniques Biology Bicuculline Inhibitory postsynaptic potential Baclofen/pharmacology Choline O-Acetyltransferase 03 medical and health sciences Prosencephalon Gamma-Aminobutyric Acid/pharmacology/ physiology medicine Animals GABA-A Receptor Agonists GABA-A Receptor Antagonists Cholinergic neuron Reversal potential 030304 developmental biology Excitatory Postsynaptic Potentials/drug effects/physiology Excitatory Postsynaptic Potentials Receptors GABA-A/agonists/antagonists & inhibitors/ physiology Receptors GABA-A Glutamate Decarboxylase/metabolism ddc:616.8 2-Amino-5-phosphonovalerate Receptors GABA-B nervous system GABA-B Receptor Agonists Synaptic Transmission/drug effects/physiology Biophysics Cholinergic Choline O-Acetyltransferase/ metabolism GABA-B Receptor Antagonists Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience Neuroscience, Elsevier-International Brain Research Organization, 1998, 86 (3), pp.937-947. ⟨10.1016/s0306-4522(98)00094-3⟩ Neuroscience, Vol. 86, No 3 (1998) pp. 937-947 |
| ISSN: | 0306-4522 1873-7544 |
| DOI: | 10.1016/s0306-4522(98)00094-3 |
| Popis: | The potential influence of GABAergic input to cholinergic basalis neurons was studied in guinea-pig basal forebrain slices. GABA and its agonists were applied to electrophysiologically-identified cholinergic neurons, of which some were labelled with biocytin and confirmed to be choline acetyltransferase-immunoreactive. Immunohistochemistry for glutamate decarboxylase was also performed in some slices and revealed GABAergic varicosities in the vicinity of the biocytin-filled soma and dendrites of electrophysiologically-identified cholinergic cells. From rest (average - 63 mV), the cholinergic cells were depolarized by GABA. The depolarization was associated with a decrease in membrane resistance and diminution in firing. The effect was mimicked by muscimol, the specific agonist for GABA(A) receptors, and not by baclofen, the specific agonist for GABA(B) receptors, which had no discernible effect. The GABA- and muscimol-evoked depolarization and decrease in resistance were found to be postsynaptic since they persisted in the presence of solutions containing either high Mg2+/low Ca2+ or tetrodotoxin. They were confirmed as being mediated by a GABA(A) receptor, since they were antagonized by bicuculline. The reversal potential for the muscimol effect was estimated to be approximately -45 mV, which was -15 mV above the resting membrane potential. Finally, in some cholinergic cells, spontaneous subthreshold depolarizing synaptic potentials (average 5 mV in amplitude), which were rarely associated with action potentials, were recorded and found to persist in the presence of glutamate receptor antagonists but to be eliminated by bicuculline. These results suggest that GABAergic input may be depolarizing, yet predominantly inhibitory to cholinergic basalis neurons. |
| Databáze: | OpenAIRE |
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