Discovery of Hyperactive Antifreeze Protein from Phylogenetically Distant Beetles Questions Its Evolutionary Origin
| Autor: | Yuji C. Sasaki, Yoshiyuki Nishimiya, Tatsuya Arai, Akari Yamauchi, Sakae Tsuda, Hidemasa Kondo, Ai Miura |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Stag beetle lcsh:Chemistry Antifreeze Proteins Hemolymph Freezing Protein Isoforms thermal hysteresis (TH) Tenebrio lcsh:QH301-705.5 Phylogeny Spectroscopy Genetics biology General Medicine Biological Evolution Computer Science Applications Coleoptera tandem repeat Ice binding Larva Insect Proteins stag beetle antifreeze protein (AFP) Signal peptide Gene isoform evolutionary origin Article Catalysis Evolution Molecular Inorganic Chemistry 03 medical and health sciences freeze avoidance Tandem repeat Antifreeze protein Consensus sequence Animals Amino Acid Sequence Dorcus Physical and Theoretical Chemistry ice binding Molecular Biology Base Sequence 030102 biochemistry & molecular biology Organic Chemistry supercooling biology.organism_classification digestive system diseases 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 3637, p 3637 (2021) International Journal of Molecular Sciences Volume 22 Issue 7 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Beetle hyperactive antifreeze protein (AFP) has a unique ability to maintain a supercooling state of its body fluids, however, less is known about its origination. Here, we found that a popular stag beetle Dorcus hopei binodulosus (Dhb) synthesizes at least 6 isoforms of hyperactive AFP (DhbAFP). Cold-acclimated Dhb larvae tolerated −5 °C chilled storage for 24 h and fully recovered after warming, suggesting that DhbAFP facilitates overwintering of this beetle. A DhbAFP isoform (~10 kDa) appeared to consist of 6−8 tandem repeats of a 12-residue consensus sequence (TCTxSxNCxxAx), which exhibited 3 °C of high freezing point depression and the ability of binding to an entire surface of a single ice crystal. Significantly, these properties as well as DNA sequences including the untranslated region, signal peptide region, and an AFP-encoding region of Dhb are highly similar to those identified for a known hyperactive AFP (TmAFP) from the beetle Tenebrio molitor (Tm). Progenitor of Dhb and Tm was branched off approximately 300 million years ago, so no known evolution mechanism hardly explains the retainment of the DNA sequence for such a long divergence period. Existence of unrevealed gene transfer mechanism will be hypothesized between these two phylogenetically distant beetles to acquire this type of hyperactive AFP. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|