Mouse neutrophils release extracellular traps in response to Naegleria fowleri
| Autor: | Rafael Campos-Rodríguez, Israel López-Reyes, Patricia Bonilla-Lemus, Diana Falcon-Acosta, Judith Pacheco-Yépez, Arturo Contis-Montes de Oca, Erika Rosales-Cruz, María Maricela Carrasco-Yépez, Itzel Berenice Rodriguez-Mera, Saúl Rojas-Hernández |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Extracellular Traps Neutrophils 030231 tropical medicine Immunology Mucous membrane of nose Central Nervous System Protozoal Infections Microbiology Histones 03 medical and health sciences Mice 0302 clinical medicine In vivo parasitic diseases medicine Animals Humans Trophozoites Cells Cultured Naegleria fowleri Peroxidase Mice Inbred BALB C Microscopy Confocal biology Neutrophil extracellular traps Amebiasis DNA Protozoan biology.organism_classification In vitro Coculture Techniques Nasal Mucosa 030104 developmental biology medicine.anatomical_structure Myeloperoxidase biology.protein Parasitology Bone marrow |
| Zdroj: | Parasite immunology. 41(2) |
| ISSN: | 1365-3024 |
| Popis: | Naegleria fowleri is a free-living amoeba, which is able to infect humans through the nasal mucosa causing a disease in the central nervous system known as primary amoebic meningoencephalitis (PAM). Polymorphonuclear cells (PMNs) play a critical role in the early phase of N fowleri infection. Recently, a new biological defence mechanism called neutrophil extracellular traps (NETs) has been attracting attention. These structures represent an important strategy to immobilize and kill invading microorganisms. In this work, we evaluate the capacity of N fowleri to induce the NETs release by PMNs cells in mice in vitro and in vivo. In vitro: Neutrophils from bone marrow were cocultured with N fowleri trophozoites. In vivo: we employed a mouse model of PAM. We evaluated DNA, histone and myeloperoxidase (MPO) and the formation of NETs by confocal microscopy. Our results showed N fowleri induce both NETs and MPO release by PMNs cells in mice after trophozoite exposure, which increased through time, in vitro and in vivo. These results demonstrate that NETs are somehow associated with the amoebas. We suggest PMNs release their traps trying to avoid N fowleri attachment at the apical side of the nasal epithelium. |
| Databáze: | OpenAIRE |
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