Low-cost HPV testing and the prevalence of cervical infection in asymptomatic populations in Guatemala
| Autor: | Victor Argueta, Enrique Alvirez, Joël Fokom Domgue, Femke J. H. B. van Aerde-van Nunen, Eduardo Gharzouzi, Roberto Orozco, Jami Troxler, Miriam Castillo, Heidi McMillen, Herbert M. Pinedo, Hesler Morales, Héctor Rosas, Bailey Kessing, Hong Lou, Andert Rosingh, Griselda Lopez, Mark Schiffman, Joseph Boland, Sarah Wagner, Lisa Garland, Sarita Polo Guerra, Julie Sawitzke, Michael Dean, Guillermo Villagran |
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| Rok vydání: | 2018 |
| Předmět: |
HPV
Cancer Research medicine.medical_specialty lcsh:RC254-282 Asymptomatic 03 medical and health sciences 0302 clinical medicine Surgical oncology Cytology Epidemiology Prevalence Genetics medicine 030212 general & internal medicine Cervical cancer Obstetrics business.industry virus diseases Guatemala lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease female genital diseases and pregnancy complications Hpv testing Real-time polymerase chain reaction Oncology 030220 oncology & carcinogenesis Screening Population study medicine.symptom business Real-time PCR |
| Zdroj: | BMC Cancer, Vol 18, Iss 1, Pp 1-7 (2018) |
| ISSN: | 1471-2407 |
| Popis: | Background A low cost and accurate method for detecting high-risk (HR) human papillomavirus (HPV) is important to permit HPV testing for cervical cancer prevention. We used a commercially available HPV method (H13, Hybribio) which was documented to function accurately in a reduced volume of cervical specimen to determine the most prevalent HPV types and the distribution of HPV infections in over 1795 cancer-free women in Guatemala undergoing primary screening for cervical cancer by cytology. Methods HR-HPV detection was attempted in cervical samples from 1795 cancer-free women receiving Pap smears using the Hybribio™ real-time PCR assay of 13 HR types. The test includes a globin gene internal control. HPV positive samples were sequenced to determine viral type. Age-specific prevalence of HPV was also assessed in the study population. Results A total of 13% (226/1717) of women tested HPV+, with 78 samples (4.3%) failing to amplify the internal control. The highest prevalence was found in younger women ( |
| Databáze: | OpenAIRE |
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