An Activity-Based Fluorescent Sensor for the Detection of the Phenol Sulfotransferase SULT1A1 in Living Cells
| Autor: | Jasmine N. Tutol, Sheena D'Arcy, Kierstin M Page, Regina A. Baglia, Steven O. Nielsen, Sheel C. Dodani, Jyothi Kallu, Koushambi Mitra, Sriharika Gottipolu, Kira R Mills, Darby Ball |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Gene isoform Cell signaling Sulfotransferase 010402 general chemistry 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Biochemistry 03 medical and health sciences chemistry.chemical_compound Moiety Solubility Sulfuryl Molecular Biology biology Chemistry Substrate (chemistry) Metabolism Fluorescence Enzyme assay In vitro 0104 chemical sciences 030104 developmental biology Chemistry (miscellaneous) biology.protein |
| Zdroj: | Rsc Chemical Biology |
| DOI: | 10.26434/chemrxiv.13215167.v1 |
| Popis: | Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here, we present the development, computational modeling, in vitro enzymology, and biological application of STS-3, an activity-based fluorescent sensor for the SULT1A1 isoform. Here, we report the development, computational modeling, in vitro enzymology, and biological application of an activity-based fluorescent sensor for the human phenol sulfotransferase SULT1A1. |
| Databáze: | OpenAIRE |
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