Contraction of collagen lattices by cells from Dupuytren's nodules
| Autor: | S. Wingren, M. R. Ghassemifar, E. Tarpila, L. Franzén, M. Ågren |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Pathology
medicine.medical_specialty Cell type Platelet-derived growth factor medicine.medical_treatment Becaplermin Biology chemistry.chemical_compound Dermis Cell Movement medicine Animals Humans Fibroblast Cells Cultured Platelet-Derived Growth Factor Transplantation Microscopy Confocal integumentary system Growth factor Proto-Oncogene Proteins c-sis Fibroblasts Flow Cytometry Culture Media Rats body regions Dupuytren Contracture medicine.anatomical_structure Cytokine chemistry Cell culture Connective Tissue Surgery Collagen Contracture medicine.symptom |
| Zdroj: | Journal of hand surgery (Edinburgh, Scotland). 21(6) |
| ISSN: | 0266-7681 |
| Popis: | The aim of this study was to see if nodular cells in Dupuytren’s disease differed from dermal cells in their contractile capacity and motility. Ten surgical specimens from patients with Dupuytren’s disease and contracture of the finger of more than 45° were harvested and the nodular cells were explanted and cultured. Dermal fibroblasts from the forearm were used as control cells. Both types of cell had the same growth pattern. The morphology on confocal laser scanning microscopy was also similar in both types of cell. Dermal control cells caused significantly more contraction of collagen lattices compared with fibroblasts from nodules of Dupuytren’s contracture. The F-actin content was equal in both groups. Platelet derived growth factor, PDGF-BB (but not PDGF-AA), increased the chemotactic activity of both cell types, but there were no differences between them. The results indicate that at a late state of the disease cells from Dupuytren’s nodules lose their contractile capacity and regain a phenotype resembling that of dermal fibroblasts. |
| Databáze: | OpenAIRE |
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