In vitro genotoxic and cytotoxic effects of doxepin and escitalopram on human peripheral lymphocytes
| Autor: | Hayal Cobanoglu, Mahmut Coskun, Munevver Coskun, Akin Cayir |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Survival Health Toxicology and Mutagenesis Serotonin reuptake inhibitor Sister chromatid exchange Pharmacology Citalopram Antidepressive Agents Tricyclic Toxicology medicine.disease_cause Risk Assessment 03 medical and health sciences 0302 clinical medicine medicine Escitalopram Humans Lymphocytes Micronuclei Chromosome-Defective Chemical Health and Safety Dose-Response Relationship Drug Chemistry Public Health Environmental and Occupational Health General Medicine Doxepin Comet assay 030104 developmental biology 030220 oncology & carcinogenesis Antidepressive Agents Second-Generation Micronucleus Sister Chromatid Exchange Genotoxicity Selective Serotonin Reuptake Inhibitors medicine.drug DNA Damage Mutagens |
| Zdroj: | Drug and chemical toxicology. 41(2) |
| ISSN: | 1525-6014 |
| Popis: | Antidepressants are drugs used for the treatment of many psychiatric conditions including depression. There are findings suggesting that these drugs might have genotoxic, carcinogenic, and/or mutagenic effects. Therefore, the present in vitro study is intended to investigate potential genotoxic and cytotoxic effects of the antidepressants escitalopram (selective serotonin reuptake inhibitor) and doxepin (Tricyclic antidepressant) on human peripheral lymphocytes cytokinesis-block micronucleus (CBMN), sister chromatid exchange (SCE), and single cell gel electrophoresis (alkaline comet assay) were used for the purpose of the study. In the study, four different concentrations of both drugs (1, 2.5, 5, and 10 µg/mL) were administered to human peripheral lymphocytes for 24 h. The tested concentrations of both drugs were found to exhibit no cytotoxic and mitotic inhibitory effects. SCE increase caused by 5 and 10 µg/mL of escitalopram was found statistically significant, while no statistically significant increase was observed in DNA damage and micronucleus (MN) formation. Moreover, the increase caused by doxepin in MN formation was not found statistically significant. Besides, 10 µg/mL of doxepin was demonstrated to significantly increase arbitrary unit and SCE formation. These findings suggest that the investigated concentrations of escitalopram and doxepin were non-cytotoxic but potentially genotoxic at higher concentrations. |
| Databáze: | OpenAIRE |
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