Direct double antibody sandwich immunoassay for Pseudomonas aeruginosa elastase
| Autor: | M.-C. Jaffar-Bandjee, O. Guy-Crotte, A. Lazdunski, C. Galabert, J. Carrere |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Detection limit
Cystic Fibrosis Pancreatic Elastase biology medicine.diagnostic_test Pseudomonas aeruginosa Chemistry Immunology Elastase Enzyme-Linked Immunosorbent Assay biology.organism_classification medicine.disease_cause Molecular biology Horseradish peroxidase Immunoassay Pseudomonadales biology.protein medicine Humans Immunology and Allergy Antibody Pseudomonadaceae |
| Zdroj: | Journal of Immunological Methods. 164:27-32 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(93)90272-9 |
| Popis: | A direct sandwich enzyme immunoassay was developed in order to quantify Pseudomonas aeruginosa elastase. As a solid phase the wells of a microtitre plate were coated with specific IgG and horseradish peroxidase labelled IgG was used as the second antibody. The detection limit of the assay was 0.26 ng/ml and a good agreement was found with elastolytic activity determined using elastin-Congo red. This assay was simple, specific, sensitive and reproducible, and permits the determination of low levels of elastase. |
| Databáze: | OpenAIRE |
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