An initial examination of the potential role of T-cell immunity in protection against feline immunodeficiency virus (FIV) infection
| Autor: | Janet K. Yamamoto, Jennifer L. Owen, Ruiyu Pu, Alek M Aranyos, James K. Coleman, Shannon R. Roff |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Interleukin 2 Feline immunodeficiency virus viruses animal diseases T-Lymphocytes Immunodeficiency Virus Feline Antibodies Viral Virus Article 03 medical and health sciences Interferon-gamma 0302 clinical medicine Immune system Immunity Feline Acquired Immunodeficiency Syndrome medicine Animals 030212 general & internal medicine B-Lymphocytes Immunity Cellular General Veterinary General Immunology and Microbiology biology Histocompatibility Antigens Class I Vaccination Public Health Environmental and Occupational Health virus diseases Viral Vaccines biochemical phenomena metabolism and nutrition biology.organism_classification Virology Adoptive Transfer Antibodies Neutralizing 030104 developmental biology Infectious Diseases Granzyme Immunology biology.protein Cats Molecular Medicine Interleukin-2 medicine.drug |
| Popis: | The importance of vaccine-induced T-cell immunity in conferring protection with prototype and commercial FIV vaccines is still unclear. Current studies performed adoptive transfer of T cells from prototype FIV-vaccinated cats to partial-to-complete feline leukocyte antigen (FLA)-matched cats a day before either homologous FIVPet or heterologous-subtype pathogenic FIVFC1 challenge. Adoptive-transfer (A-T) conferred a protection rate of 87% (13 of 15, p0.001) against FIVPet using the FLA-matched T cells, whereas all 12 control cats were unprotected. Furthermore, A-T conferred protection rate of 50% (6 of 12, p0.023) against FIVFC1 using FLA-matched T cells, whereas all 8 control cats were unprotected. Transfer of FLA-matched T and B cells demonstrated that T cells are needed to confer A-T protection. In addition, complete FLA-matching and addition of T-cell numbers13 × 10(6) cells were required for A-T protection against FIVFC1 strain, reported to be a highly pathogenic virus resistant to vaccine-induced neutralizing-antibodies. The addition of FLA-matched B cells alone was not protective. The poor quality of the anti-FIV T-cell immunity induced by the vaccine likely contributed to the lack of protection in an FLA-matched recipient against FIVFC1. The quality of the immune response was determined by the presence of high mRNA levels of cytolysin (perforin) and cytotoxins (granzymes A, B, and H) and T helper-1 cytokines (interferon-γ [IFNγ] and IL2). Increased cytokine, cytolysin and cytotoxin production was detected in the donors which conferred protection in A-T studies. In addition, the CD4(+) and CD8(+) T-cell proliferation and/or IFNγ responses to FIV p24 and reverse transcriptase increased with each year in cats receiving 1X-3X vaccine boosts over 4 years. These studies demonstrate that anti-FIV T-cell immunity induced by vaccination with a dual-subtype FIV vaccine is essential for prophylactic protection against AIDS lentiviruses such as FIV and potentially HIV-1. |
| Databáze: | OpenAIRE |
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