Reduced Dendritic Spines in the Visual Cortex Contralateral to the Optic Nerve Crush Eye in Adult Mice
| Autor: | Zitian Liu, Lianyan Huang, Yadan Quan, Yali Wu, Kaili Wu, Deling Li, Shana Yang, Zongyi Zhan, Yiru Huang, Minbin Yu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Dendritic spine genetic structures Dendritic Spines Blotting Western Optic Disk Nerve fiber layer Tropomyosin receptor kinase B Crush Injuries Mice 03 medical and health sciences 0302 clinical medicine Microscopy Electron Transmission medicine Animals Immunoprecipitation primary visual cortex Visual Cortex Glial fibrillary acidic protein biology Microglia Chemistry Optic Nerve optic nerve crush Oligodendrocyte Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure nervous system Optic Nerve Injuries biology.protein Optic nerve Evoked Potentials Visual Female sense organs Visual Neuroscience NeuN Tomography Optical Coherence 030217 neurology & neurosurgery |
| Zdroj: | Investigative Ophthalmology & Visual Science |
| ISSN: | 1552-5783 |
| DOI: | 10.1167/iovs.61.10.55 |
| Popis: | Purpose To determine alteration of dendritic spines and associated changes in the primary visual cortex (V1 region) related to unilateral optic nerve crush (ONC) in adult mice. Methods Adult unilateral ONC mice were established. Retinal nerve fiber layer (RNFL) thickness was measured by spectral-domain optical coherence tomography. Visual function was estimated by flash visual evoked potentials (FVEPs). Dendritic spines were observed in the V1 region contralateral to the ONC eye by two-photon imaging in vivo. The neurons, reactive astrocytes, oligodendrocytes, and activated microglia were assessed by NeuN, glial fibrillary acidic protein, CNPase, and CD68 in immunohistochemistry, respectively. Tropomyosin receptor kinase B (TrkB) and the markers in TrkB trafficking were estimated using western blotting and co-immunoprecipitation. Transmission electron microscopy and western blotting were used to evaluate autophagy. Results The amplitude and latency of FVEPs were decreased and delayed at 3 days, 1 week, 2 weeks, and 4 weeks after ONC, and RNFL thickness was decreased at 2 and 4 weeks after ONC. Dendritic spines were reduced in the V1 region contralateral to the ONC eye at 2, 3, and 4 weeks after ONC, with an unchanged number of neurons. Reactive astrocyte staining was increased at 2 and 4 weeks after ONC, but oligodendrocyte and activated microglia staining remained unchanged. TrkB was reduced with changes in the major trafficking proteins, and enhanced autophagy was observed in the V1 region contralateral to the ONC eye. Conclusions Dendritic spines were reduced in the V1 region contralateral to the ONC eye in adult mice. Reactive astrocytes and decreased TrkB may be associated with the reduced dendritic spines. |
| Databáze: | OpenAIRE |
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