A mixture of honey bee products ameliorates the genotoxic side effects of cyclophosphamide
Autor: | Nagwa H. Hassan, Heba Gamal Elalfy, Maha A. Fahmy, Samia A. El-Fiky |
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Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Microbiology (medical)
Protective role Honey bee food.ingredient lcsh:Arctic medicine. Tropical medicine Cyclophosphamide lcsh:RC955-962 Royal jelly lcsh:Medicine Pharmacology Biology medicine.disease_cause Toxicology food Oral administration medicine lcsh:R food and beverages Pollen grains Sperm Infectious Diseases medicine.anatomical_structure DNA fragmentation Bone marrow Genotoxicity medicine.drug |
Zdroj: | Asian Pacific Journal of Tropical Disease, Vol 5, Iss 8, Pp 638-644 (2015) |
ISSN: | 2222-1808 |
Popis: | Objective To evaluate the protective role of a mixture of honey bee products (honey, royal jelly and pollen grains) against the genotoxicity induced by the anticancer drug cyclophosphamide (CP). Methods The study included chromosomal aberration analysis in mice bone marrow cells, induction of morphological sperm abnormalities, DNA fragmentation and histopathological changes induced in liver cells of mice. CP was injected intraperitoneally at the dose of 20 mg/kg body weight. The mixture of honey bee products was administrated orally for different periods of time 5, 10 and 15 days with a dose exactly equivalent to the daily intake of human beings. Results The results revealed that honey mixture ameliorated the genotoxic side effects of CP. For chromosomal aberrations the percentage reached 25.20 ± 1.30 for CP treated group, while it reached half of that value 12.30 ± 0.54 in CP-group pretreated with honey mixture for 15 days. Breaks, fragments and multiple aberrations were the most pronounced types of aberrations induced after CP treatment and honey mixture reduced these types of abnormalities. CP induced significant percentage of sperm abnormalities 8.52 ± 0.17 compared to control 3.10 ± 0.10. The percentage of sperm abnormalities reached nearly to the control value in CP- mice treated with honey mixture for 15 days. Honey also reduced the incidence of liver DNA damage induced by CP. The results also indicated that CP had a marked damaging effect on liver tissue including severe dilatation, congestion of main blood vessels and massive infiltration of inflammatory cells with irregular general pattern of the tissue. These effects were greatly ameliorated by using oral administration of honey mixture for different periods of time. Conclusions The results concluded that honey bee mixture can be used as chemopreventive agent for minimizing the genotoxic side effects of the anticancer drug CP and open the field for its use in many applications. |
Databáze: | OpenAIRE |
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