Effects of sample storage and diluents in the reliability of thyroglobulin measurement in the washout of fine needle aspirates
| Autor: | Alicia Lowenstein, Fabián Pitoia, Patricia Glikman, Fanny Piotrkowski-Viale, Adriana Reyes, Alicia Dios |
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| Rok vydání: | 2017 |
| Předmět: |
Thyroid nodules
endocrine system medicine.medical_specialty endocrine system diseases Endocrinology Diabetes and Metabolism medicine.medical_treatment Biopsy Fine-Needle 030209 endocrinology & metabolism Thyroid Function Tests Sensitivity and Specificity Thyroglobulin Specimen Handling Thyroid carcinoma 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine medicine Humans Thyroid Neoplasms Thyroid Nodule Saline Thyroglobulin Measurement Chemistry Washout Reproducibility of Results medicine.disease Calcitonin 030220 oncology & carcinogenesis Lymph hormones hormone substitutes and hormone antagonists |
| Zdroj: | Endocrine. 56(3) |
| ISSN: | 1559-0100 |
| Popis: | To evaluate two variables affecting Thyroglobulin stability in the washout of fine needle aspiration biopsies of thyroid nodules and metastatic lymph nodes. Thyroglobulin stability after storage at −20 °C up to 14 days was studied in washout performed with normal saline solution and further dilutions with normal saline solution from five metastatic thyroid nodes and six benign thyroid nodules. We also studied thyroglobulin stability in diluents compared with normal saline solution: 4% bovine serum albumin in normal saline solution and diluents free from thyroglobulin or Calcitonin or parathyroid hormone in paired and simultaneous thyroglobulin measurements of washout dilutions from 5/6 benign thyroid nodules and 2/5 metastatic lymph nodes. Thyroglobulin and Thyroglobulin antibodies were measured by a chemiluminescent assay. Positive samples with thyroglobulin antibodies in serum and/or washout were excluded. Thyroglobulin decreased with storage in washout or dilutions of washout performed in normal saline solution (p |
| Databáze: | OpenAIRE |
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