Rapid Diagnosis of Clonal Immunoglobulin Heavy Chain Gene Rearrangements in Cutaneous B-Cell Lymphomas Using the LightCycler-Polymerase Chain Reaction with DNA Melting Curve Analysis
| Autor: | Juan Du, Dongsheng Xu, Hideko Kamino, Howard Ratech |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Lymphoma B-Cell Skin Neoplasms Chronic lymphocytic leukemia Gene Rearrangement B-Lymphocyte Heavy Chain Cutaneous B-cell lymphoma Dermatology Biology Polymerase Chain Reaction Sensitivity and Specificity Pathology and Forensic Medicine law.invention Nucleic acid thermodynamics immune system diseases law hemic and lymphatic diseases medicine Humans Transition Temperature Prospective Studies False Negative Reactions Polymerase chain reaction B cell Aged Aged 80 and over Infant DNA General Medicine Gene rearrangement Middle Aged medicine.disease Molecular biology Clone Cells Lymphoma medicine.anatomical_structure Immunoglobulin heavy chain Electrophoresis Polyacrylamide Gel Female |
| Zdroj: | The American Journal of Dermatopathology. 26:385-389 |
| ISSN: | 0193-1091 |
| DOI: | 10.1097/00000372-200410000-00007 |
| Popis: | We have recently developed a novel Immunoglobulin heavy chain gene rearrangement (IgH-R) assay that combines polymerase chain reaction (PCR) amplification and analysis in the same closed capillary tube using the LightCycler System. IgH-R can be identified by DNA melting curve analysis within 40 minutes after DNA preparation and amplification. To test the clinical utility of this new IgH-R assay for rapidly diagnosing cutaneous B-cell lymphomas, we prospectively analyzed 44 formalin-fixed, paraffin-embedded tissues suspected of B-cell malignant lymphoma: skin (n = 31), lymph node (n = 7), stomach (n = 3), spleen (n = 1), colon (n = 1), and soft tissue (n = 1). We detected IgH-R in 12 DNA samples, including 8 skin biopsies, with the following diagnoses: B-cell chronic lymphocytic leukemia (n = 4), extranodal marginal zone B-cell lymphoma (n = 4), diffuse large B-cell lymphoma (n = 2), Burkitt lymphoma (n = 1), and precursor B-lymphoblastic lymphoma (n = 1). DNA melting curve analysis, compared with polyacrylamide gel electrophoresis, achieved a sensitivity equal to 92.3% and a specificity equal to 100%. There was a single false negative result because DNA melting curve analysis could not detect less than 10.0% clonal B-cells. We conclude that this new, rapid PCR assay for detecting IgH-R based on DNA melting curve analysis can be clinically useful for confirming the initial diagnosis of B-cell malignant lymphoma. |
| Databáze: | OpenAIRE |
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